Histone H3 (human, recombinant) 10263 from Cayman Chemical

Product Specs
ItemHistone H3 (human, recombinant)
CompanyCayman Chemical
Price $615.00Supplier Page View Company Product Page
Catalog Number10263
Size1 mg
Specieshuman
Source/Expression SystemRecombinant human histone H3 expressed in E. coli
NCBI Full Gene NameH3 clustered histone 1
NCBI Gene AliasesH3/A, H3C10, H3C11, H3C12, H3C2, H3C3, H3C4, H3C6, H3C7, H3C8, H3FA, HIST1H3A
Research AreaEpigenetics, Transcription, & Translation, Histones/Histone Peptides, Immunology & Inflammation, Innate Immunity, NETosis
FormatA solution in water (frozen)
Molecular Weight15.5 kDa
Purity≥95% estimated by SDS-PAGE
Storage-80
OriginUSA

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Cayman Chemical

Cayman Chemical Company 1180 East Ellsworth Road

Ann Arbor, Michigan 48108

United States

Phone: (800) 364-9897
(734) 971-3640

Company Profile

Website: http://www.caymanchem.com/

(1) Preclinical characterization of AZD1163, a first-in-class anti-PAD2/4 bispecific antibody for the treatment of rheumatoid arthritisMAbsApril 15, 2026Gary P. Sims, Lacie Scaletta, John Andrews, Dorothy A. Sims, Martin Strain, Anna Sigurdardottir, Teneema Kuriakose, Elizabeth England, Lichchavi Rajasinghe, Fanyi Jiang, et al.fluids. For the measurement of recombinant PAD activity, 50 µL of a solution of Histone H3 (Cayman, #10263) at 2 µg/mL in HBSS buffer was coated on to Black High Binding plates (Greiner, #781077) for 16 hours

(2) Scaffolding of the H4K5ac chromatin remodeling complex by lncRNA MAHAC mediates epithelial-mesenchymal transitionNucleic Acids ResFebruary 10, 2026Kai-Wen Hsu, Jeng-Shou Chang, Joseph Chieh-Yu Lai, Li-Hao Yang, Pei-Hua Peng, Tzu-Chin Lin, Kou-Juey Wuinstructions. To detect CBP activity by Western blotting, human recombinant Histone H4 and H3 (No. 10263, Cayman Chemical) were included in the experiment. The acetylation reaction conditions were identical

(3) Epstein-Barr virus reprograms autoreactive B cells as antigen-presenting cells in systemic lupus erythematosusScience Translational MedicineNovember 12, 2025Shady Younis, Salvinaz I. Moutusy, Sajede Rasouli, Shaghayegh Jahanbani, Mahesh Pandit, Xiaohao Wu, Suman Acharya, Orr Sharpe, Tilini U. Wijeratne, Marlayna L. Harris, et al.Cat#11691112001), La/SSB (SURMODICS, Cat# A12801), PCNA (SURMODICS, Cat# A15401) Histone H3 (Cayman, Cat# 10263), EBNA1 (Abcam, Cat# ab138345), or LPS (Invitrogen, Cat# 00-4976-93) in carbonate-bicarbonate buffer

(4) Subjects at-risk for future development of rheumatoid arthritis demonstrate a PAD4- and TLR-dependent enhanced histone H3 citrullination and proinflammatory cytokine production in CD14hi monocytesJournal of AutoimmunityFebruary 1, 2021Yuko Okamato et al.μg of protein per well). A positive control protein was run with each blot that included H3 (Cayman, 10263), PAD4 (Cayman, 10500), and PAD2 (Cayman, 10785). Antibodies were used to detect total H3 (Abcam, ab1791),

(5) Citrullinated Histone H3 Mediates Sepsis-Induced Lung Injury Through Activating Caspase-1 Dependent Inflammasome PathwayFrontiers in immunologyJanuary 1, 2021Tian Y, Li P, Wu Z, Deng Q, Pan B, Stringer KA, Alam HB, Standiford TJ, Li Y.next day, cells were treated with 30 µg/ml CitH3 (Cayman chemical, #17926) or H3 (Cayman chemical, #10263) protein dissolved in opti-MEM medium for 5 h. Cell-free supernatant was reserved for ELISA. Cell lysates

(6) Quantification of citrullinated histones: Development of an improved assay to reliably quantify nucleosomal H3Cit in human plasmaJournal of Thrombosis and HaemostasisOctober 1, 2020Charlotte Thålin et al.described.43 Briefly, human recombinant PAD4 (Cayman #10500) and human recombinant histone H3 (Cayman #10263) were mixed at 2.5 U of PAD4 per microgram of histone and incubated at 37°C for 1 hour in reaction buffer

(7) Validation of an enzyme-linked immunosorbent assay for the quantification of citrullinated histone H3 as a marker for neutrophil extracellular traps in human plasmaImmunologic ResearchJune 1, 2017Charlotte Thålin, Maud Daleskog, Sophie Paues Göransson, Daphne Schatzberg, Julie Lasselin, Ann-Charlotte Laska, Anders Kallner, Thomas Helleday, Håkan Wallén, Mélanie Demersrecombinant PAD4 (Cayman Chemical, Cat. No. 10500), human recombinant histone H3 (Cayman Chemical, Cat. No. 10263), ELISA reader (Tecan Sunrise) Preparation of standard A working stock solution of H3Cit was made

(8) Rho GTPase-independent regulation of mitotic progression by the RhoGEF Net1Molecular biology of the cellSeptember 1, 2013Menon S, Oh W, Carr HS, Frost JA.reactions in 20 mM Tris-HCl, pH 8.0, 10 mM MgCl2, 1 mM DTT, 100 µM ATP, and 2 μg of recombinant histone H3 (10263; Cayman Chemical Company, Ann Arbor, MI) for 30 min at 30°C and stopped by addition of 5× LSB. Supernatants

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Histone H3 (human, recombinant) from Cayman Chemical

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Histone H3 (human, recombinant) from Cayman Chemical

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