Digitonin from Promega

Product Specs
ItemDigitonin
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberG9441
Quantity40microl

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) PTEN restrains SHH medulloblastoma growth through cell autonomous and nonautonomous mechanismseLifeApril 17, 2026Zhimin Lao, Salsabiel El Nagar, Yinwen Liang, Daniel N Stephen, Alexandra L Joynercompound, drug",Tamoxifen,Sigma Aldrich,Cat# T5648-1G, "Chemical compound, drug",Digitonin,Promega,Cat# G9441, "Chemical compound, drug",EdU,Invitrogen,Cat# E10187, "Chemical compound, drug",BrdU,Sigma-Aldrich,Cat#B9285-250MG, "Chemical

(2) Transient SUMOylation inhibition in human pre-adipocytes stably imprints a transcriptional beiging fateNucleic Acids ResMarch 19, 2026Patrizia Maria Christiane Nothnagel, Paul-Arthur Meslin, Jonas Aakre Wik, Damien Dufour, Yunna Erika Strøm, Magnar Bjørås, Jorrit Martijn Enserink, Bjørn Steen Skålhegg, Nolwenn Briand, Anthony Mathelier, et al.pH 7.5, 10 mM NaCl, 3 mM MgCl2 (Sigma, M1028), 0.1% NP-40, 0.1% tween 20, 0.01% Digitonin (Promega, #G9441)] and incubated on ice for 3 min. The nuclei were then washed with the ATAC wash buffer (10 mM Tris–HCl,

(3) Single cell multiomic analysis of the impact of Delta-9-tetrahydrocannabinol on HIV infected CD4 T cellsJournal of Cannabis ResearchMarch 5, 2026Manickam Ashokkumar, Renee Y. Ge, Alicia Cooper-Volkheimer, David M. Margolis, Quefeng Li, Yuchao Jiang, David M. Murdoch, Edward P. Browne655205-250ML), 0.01% nonidet P40 substitute (Sigma, cat. no. I8896), 0.01% Digitonin (Promega, cat. no. G9441), 1 U/μl RiboLock RNase inhibitor, 1% BSA and 1 mM DTT) and incubated on ice for 3 min. The released

(4) Sexual dimorphism in the cell number of the adult Drosophila brainPLOS OneFebruary 18, 2026Narendra Pratap Singh, Susan T. HarbisonATAC-RSBT1 was replaced by 500 μl of ATAC-RSBT2 (RSBT1 + 0.1% NP40, 0.1% Tween-20, and 0.01% Digitonin (G9441, Promega, Madison, USA) added on the day used). The brains were then transferred to a 2 ml Dounce homogenizer

(5) Manipulation of Alternative Splicing of IKZF1 Elicits Distinct Gene Regulatory Responses in T CellsCellsJanuary 24, 2026Lucia Pastor, Jeremy R. B. Newman, Colin M. Callahan, Rebecca R. Pickin, Mark A. Atkinson, Suna Onengut-Gumuscu, Patrick Concannon(Sigma-Aldrich, Burlington, MA, USA, Cat# 11332465001), and 0.01% Digitonin (Promega, Madison, WI, USA, Cat# G9441). After pelleting the cells, a transposition reaction was performed using Nextera DNA Library Prep Kit

(6) Multiomics Profiling of T-cell Leukemia and Lymphoma Enables Targeted Therapeutic DiscoveryCancer ResearchJanuary 16, 2026Aleksandr Ianevski, Kristen Nader, Julia Nguyen, Helena Sorger, Sanna Timonen, Edith Julia, Daniel Pölöske, Katrin Spirk, Christina Wagner, Dennis Jungherz, et al.buffer, 2.5 μL of Tn5 enzyme (Nextera DNA Library Preparation Kit, Illumina), 0.5 μL of 1% digitonin (#G9441, Promega), and 22 μL of nuclease-free water, as previously described (31). The reaction was incubated

(7) Single‐Nucleus Multi‐Omics Reveals Hypoxia‐Driven Angiogenic Programs and Their Epigenetic Control in Sinonasal Squamous Cell CarcinomaAdvanced ScienceJanuary 7, 2026Chaelin You, Jaewoo Park, Jung Yeon Jang, Junho Noh, Jaehyun Lee, Geunho Kwon, Myeong Sang Yu, Yoo‐Sam Chung, Seung‐Jun Lee, Keunsoo Kang, et al.11332473001), 0.1% Tween‐20 (Roche, Catalog no. 11332465001), and 0.01% digitonin (Promega, Catalog no. G9441). This cell lysis reaction was incubated on ice for 3 min, followed by the addition of 1 mL of RSB with

(8) Single cell and spatial characterization of the human pancreas reveals drivers of beta cell dysfunction in cystic fibrosisbioRxivDecember 16, 2025Hannah M. Mummey, Sierra Corban, Jacinta Lucero, Rebecca L. Melton, Madeleine Pittigher, Victoria C. Johnston, Gail H. Deutsch, Agnieszka D'Antonio-Chronowska, Allen Wang, Rebecca L. Hull-Meichle, et al.[AM9530G], 0.01% IGEPAL CA-630 [Sigma I8896], 0.01% Tween-20 [Sigma P7949], 0.001% digitonin [Promega G9441], 1 U/μL RNasin [N2515], and 1× Roche Complete, EDTA-free Protease Inhibitor Cocktail [5056489001].

(9) Protocol for transcriptomic and epigenomic analysis of JAK inhibitor sensitivity in IFN-γ-primed human macrophages using ATAC-seq and RNA-seqSTAR ProtocolsDecember 1, 2025Geunho Kwon, Junho Noh, Jaehyun Lee, Keunsoo Kang, Kyuho Kang15250061 "Tofacitinib citrate, JAK inhibitor",Sigma-Aldrich,Cat# PZ0017-5MG Digitonin,Promega,Cat# G9441 NP40,Roche,Cat# 11332473001 Tween 20,Roche,Cat# 11332465001 "1 M Tris-HCl, pH 7.5",Invitrogen,Cat#

(10) Gene regulatory network inference with popInfer reveals the dynamic regulation of hematopoietic stem cell quiescenceiScienceNovember 11, 2025Megan K. Rommelfanger, Marthe Behrends, Yulin Chen, Jonathan Martinez, Nikith Kurella, Nino Geisler, Deepthi Guturu, Martin Bens, Lingyun Xiong, Zijin Xiang, et al.7.4,Roth,4855.7 NaCl,Roth,3957.5 MgCl2,Merck,814733 Nonidet P40 Substitute,Roche,11332473001 Digitonin,Promega,G9441 Tween 20,Roche,11332465001 ,, Critical commercial assays,Critical commercial assays,Critical commercial

(11) TAF1 is required for fetal but not adult hematopoiesis in miceDevelopmental CellNovember 3, 2025Fan Liu, Jingyin Yue, Francesco Tamiro, Jun Sun, Pradeep Kumar Reddy Cingaram, Krystal Lisa Hossack, Concepcion Martinez Caja, Ye Xu, Chuan Chen, Felipe Beckedorff, et al.7.4, 10mM NaCl, 3mM MgCl2), supplemented with 0.1% NP40, 0.1% Tween 20 and 0.01% digitonin (Promega, G9441). Nuclei were pelleted and subjected to Tn5 transposase reaction using the Tagment DNA Enzyme and Buffer

(12) Transcription factor switching drives subtype-specific pancreatic cancerNature GeneticsOctober 30, 2025Shalini V. Rao, Lisa Young, Danya Cheeseman, Sean Flynn, Niklas Krebs, Dominique-Laurent Couturier, Stephanie Mack, Rebecca Brais, Jill Temple, Amy Smith, et al.added. A total of 50 μl cold ATAC–RSB containing 0.1% NP-40, 0.1% Tween-20 and digitonin 0.01% (Promega, G9441) was added to 50,000 viable cells and pipetted up and down thrice. The cells were incubated on ice for

(13) Integrative Transcriptomic and Epigenomic Profiling for Signature Identification in Coronary Artery Disease: A Pilot StudyInternational Journal of Molecular SciencesOctober 27, 2025Mario Zanfardino, Anna D’Agostino, Ilaria Leone, Katia Pane, Chiara Caselli, Danilo Neglia, Bruna Punzo, Carlo Cavaliere, Andrea Soricelli, Monica Franzeseof 1% digitonin, 22 μL of nuclease-free water) (Cat# FC-121-1030, Illumina, San Diego, CA, USA; Cat# G9441, Promega, Madison, WI, USA) was added to the cells, and the pellet was disrupted by pipetting. Transposition

(14) Sex-specific expression and function of TRIM28 during mouse primordial germ differentiationiScienceOctober 17, 2025DiRusso JA, Zhan L, Tao Y, Wang AL, Xiang X, Robbins AC, Cruz AJ, Liu W, Clark AT.Formamide (Acros Organics, 327171000), 3mM Tn5 Enzyme (Illumina, 20034197), 0.1% Digitonin (Promega, G9441), 0.1% Tween-20 (Sigma Aldrich, 11332465001), 0.1% NP-40(Sigma Aldrich, 11332473001). Reaction was processed

(15) Single‐Cell Nucleus Extraction with Cellular IndexingAdvanced ScienceSeptember 29, 2025Trinh Lam, Ana Esmeralda Gomez Martinez, Alison Su, Anna Fomitcheva‐Khartchenko, Xin Wang, Md Nazibul Islam, Paul Lum, Amy Elizabeth HerrTeknova (Teknova T0027). Digitonin solution supplied at 20 mg mL−1 in DMSO was purchased from Promega (G9441). Buffers Nuclei were isolated using ATAC‐RSB buffer[ 55 ] which was prepared by mixing 500 µL of

(16) Protocol to perform assay for chromatin-bound condensates by exploratory sequencing in mouse embryonic stem cellsSTAR ProtocolsSeptember 19, 2025Zeran Jia, Jiawei Yu, Yinqing Li,Thermo Fisher Scientific,28906 "10× PBS, pH 7.4",Thermo Fisher Scientific,AM9624 IGEPAL-630,Sigma-Aldrich,I3021 Digitonin,Promega,G9441 Glycine,Sigma-Aldrich,50046-50G Ethanol,Sigma-Aldrich,E7023-1L Tween 20,Sigma-Aldrich,P9416 "1,6-Hexanediol",Sigma-Aldrich,240117 "1

(17) A noncanonical role of glycolytic metabolites controlling the timing of mouse embryo segmentationScience AdvancesSeptember 19, 2025Hidenobu Miyazawa, Jona Rada, Nicole Prior, Paul Gerald Layague Sanchez, Emilia Esposito, Daria Bunina, Charles Girardot, Judith Zaugg, Alexander Aulehladimethyl formamide], 1.0 μl of TDE1 (Illumina, no. 15027865), 0.1 μl of 1% digitonin (Promega, no. G9441), 0.1 μl of 10% Tween 20 (Sigma-Aldrich, no. 11332465001), 0.1 μl of 10% NP-40 (Sigma-Aldrich, no. 11332473001),

(18) Simulating CD8 T cell exhaustion: A comprehensive approachiScienceJuly 18, 2025Andrea J. Manrique-Rincón, Ben Foster, Stuart Horswell, David A. Goulding, David J. Adams, Anneliese O. SpeakLife Science,Cat# 11332465001 NP40 (10%),Merck Life Science,Cat# 11332473001 Digitonin,Promega,Cat# G9441 ,, Critical commercial assays,Critical commercial assays,Critical commercial assays ,, LS Columns,Miltenyi Biotec

(19) Combined inhibition of KAT6A/B and Menin reverses estrogen receptor-driven gene expression programs in breast cancerCell Reports MedicineJuly 15, 2025Sarah Naomi Olsen, Bryn Anderson, Charlie Hatton, Zhengtao Chu, Christopher Simpkins, Yanhe Wen, Wallace Bourgeois, Elena L. Haarer, Myles Brown, Rinath Jeselsohn, et al.Tablets, EDTA-free",Roche,Cat#1873580 β-mercaptoethanol,Sigma-Aldrich,Cat#M6250 Digitonin,Promega,Cat#G9441 TWEEN-20,Roche Diagnostics,Cat#11332465001 Geneticin G418,Thermo Fisher Scientific,Cat#10131-035 PF-9363,MedChem

(20) Genome-wide rules of transcription factor cooperativity revealed through in silico binding site ablationbioRxivJune 25, 2025Xuening He, Hjorleifur Einarsson, Petra Palenikova, Sarah Rennie, Dewei Hu, Ruining Cui, Christian Vaagenso, Kasper Lage, Robert Krautz, Robin Andersson(10mM Tris-HCl, ph7.5; 10mM NaCl; 3mM MgCl2; 0.1% v/v NP-40; 0.1% v/v Tween20; 1% Digitonin [Promega, G9441]), incubated for 5 min at 4°C, sheared by taking the samples up 10 times into a 1 ml syringe assembled

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Digitonin

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Digitonin from Promega

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