Description
EnzScript Reverse Transcriptase is a Moloney Murine Leukemia Virus (M-MLV) reverse transcriptase with point mutations in the RNase H domain that eliminate detectable RNase H activity. EnzScript can be used to generate first-strand cDNA from poly(A) mRNA or total RNA for use in downstream applications such as RT-PCR, cDNA cloning or library construction for RNA-Seq. The point mutations in the RNase H domain increase the thermostability of the enzyme and support greater cDNA yield of full-length transcripts than wild type M-MLV reverse transcriptase