In order to compare the ability to inhibit ribonuclease activity, rat RNA was pre-mixed with RNaseOFF (Left image) and the leading competitor's RNase (right image) in a 20ul reaction. Increasing amoutn of RNase A enzyme ranging from 5ng to 50ng per reaction was added to into each reaction mixture. The reactions were incubated at 37°C for 15 minutes and loaded onto a 2% gel to viasualize the degree of RNA degradation. At 15ng/rxn of of RNase A, both 285 and 185 ribosomal RNA bands are clearly visible in RNaseOff Reaction (left image). However , the 285 band is degraded in the competitor's reaction (right image).