Fig 1: Fate and competition analysis of pluripotent aggregates and EpiGastruloids.a, Representative micrographs of 120 h pluripotent mosaic 3D aggregates grown in N2B27 + 2i+LIF. (corresponds to condition without LIF in Fig. 7a). b, WT-mCherry growth curves in mosaic pluripotent aggregates seeded together with WT-emiRFP (blue) or p53KO-emiRFP (dark green) cells. Mean ± SEM of n = 12 gastruloids per datapoint from 3 indep. experiments. c, Fate marker immunostaining of 120 h mosaic WT + WT (left) and WT+p53KO (right) aggregates grown either as standard gastruloids (ChIR) or remaining as pluripotent aggregates in 2i or 2i+LIF. Maximum intensity projections of confocal laser scanning Z-stacks. Micrographs depicted as composite of mCherry (green) and emiRFP (magenta), or inverted immunostaining alone (gray scale). d, e, Fate marker immunostaining of mosaic WT + WT (d) and WT+p53KO (e) EpiGastruloids at 72 h. Maximum intensity projections of spinning disk confocal Z-stacks. Micrographs depicted as composite (top panel) of mCherry (blue), emiRFP (magenta), and marker staining (yellow), or inverted grey scale marker staining alone (lower panel). f, Growth curves of cell populations in mosaic EpiGastruloids depicted as mean ± SEM. n = 17 gastruloids per datapoint from 3 indep. experiments. Scale bars denote 400 µm. Source data