Fig 1: Modulation of apoptosis in glioblastoma cells(A–D) Effects of anti-TNF inhibitory antibody (5 μg/ml), anti-TRAIL inhibitory antibody (5 μg/ml) or anti-Fas-L inhibitory antibody (5 μg/ml) in the media on CBD-induced apoptosis in U87MG and U118MG cells. Typical experiment of cell cycle apoptosis-analysis with PI-stained nuclei is shown in the panel B. The pooled results of four independent experiments are presented in panels (A–C). Error bars represent means ± S.D. (p < 0.05, Student’s t-test). Stars indicate a significant difference in CBD-induced apoptotic levels between control and anti-TNF- or anti-TRAIL-treated U87MG cells. (E) Effects of CBD (20 µM) pretreatment (18 h) on the subsequent treatment (24 h) of glioma cells with exogenous FasL (50 ng/ml) + CHX (1 µg/ml), TNFα (20 ng/ml) + CHX, and TRAIL (50 ng/ml) + CHX. Levels of apoptosis were determined using cell cycle-apoptosis analysis. Stars and black circles indicated significant differences.