Description
Human CD4 protein is also known as IL16 receptor (IL16R). CD4 dimer (CSP-24004) contains CD4 extracellular 4-domains (amino acids Lys26-Phe396, UniProt# A0A4Y5UGE4) with a homodimer motif and a His tag at the C-terminus. This protein is expressed in HEK293 cells. The CD4 dimer protein is bioactive and can bind to HIV-1 envelope glycoproteins (Env) from various subtypes with on average >10 fold binding activity increases compared to CD4 monomer protein. The HIV-1 Env binding to CD4 dimer can be diminished by a D368R mutation. CD4 dimer also increases the binding to CD4-specific antibodies. This CD4 dimer can be used as an immunogen for animal immunization (RUO) and as an antigen for in vitro assays and antibody screening targeting more conformations epitopes