Description
T7 RNA polymerase Recombinant protein is produced by bacteriophage T7 DNA which is expressed in recombinant E coli bacterial system
T7 RNA Polymerase is a DNA-dependent 5'--> 3' RNA polymerase which specifically recognizes T7 promoter sequences.
T7 RNA polymerase active enzyme synthesizes RNA at an increasing degree of that of E coli RNA polymerase and it terminates transcription often. T7 RNA polymerase is very selective for initiation at its own promoter sequences and is resistant to antibiotics that inhibit E coli RNA polymerase. In-vitro transcription of mRNA is achieved via bacteriophage T7 RNA polymerase using its ability to produce full-length RNA transcripts with high reliability, thoughT7 RNAP can manufacture as well immunostimulatory by products for example dsRNA which affect protein expression