Protein G (Agarose), Fast Flow P9102-59 from United States Biological

Protein G (Agarose), Fast Flow

Elution Profile and Analysis: Mouse IgG was purified from 2L of hybridoma supernatant fraction using Protein G Agarose (P9102-59), Fast Flow. The column eluent was monitored with an UV detector and recorded at 280nm.
Protein G (Agarose), Fast Flow

Immunoprecipitation: Representative blot from a previous lot. IP of Phosphotyrosine containing proteins contained in EGF-stimulated A431 cell lysate (4ug), in conjunction with 20ul (50% slurry) Protein G Agarose (P9102-59), fast flow followed by immunoblot analysis. Blot was developed using an HRP-conjugated secondary antibody and the ECL detection system.

Supplier Page from
United States Biological for
Protein G (Agarose), Fast Flow

Product Specs
ItemProtein G (Agarose), Fast Flow
CompanyUnited States Biological
Price $1,293.00Supplier Page View Company Product Page
Catalog NumberP9102-59
FormatSupplied as 10ml packed beads, containing 2mg/ml Protein G suspended as a 50% slurry in distilled water containing 20% ethanol for a final volume of 20ml. Binding capacity of 20mg human IgG/ml agarose. Prior to use, wash the agarose beads with an appropriate buffer to remove the ethanol
ApplicationsIP
Conjugate/TagAgarose
Size10ml