Adipogenesis inhibition of MSCs. MSCs (Mesenchymal stem cells) were maintained in DMEM, supplemented with 10% fetal bovine serum, penicilin-streptomycin and glutamine. For differentiation of MSCs, MSCs were cultured in adipogenic medium which was growth medium supplemented with 1uM Dexamethasone, 0.5mM IBMX, 10ug/m lnsulin, 100uM Indomethacin (day 1). Medium was changed every 3 days. Staining with Oil Red O was typically performed on day 30. For negative controls, TNF-α (20ng/ml) was added. To immobilize Notch ligands on the plastic surface of the culture plates, plates were first incubated with a solution of anti-FLAG® antibody (10ug/ml) in PBS for 30 mins at 37°C. Coated plates were blocked with growth medium for at least 30mins, incubated with solution of 044746 (5ug/ml) or mCD137L-FLAG® (5ug/ml) in growth medium for 2 hours at 37°C. Plates were then used to differentiate MSCs.