Affinity Precipitation/Immunoblot Analysis: Representative blot from a previous lot. Lysates of human neutrophils that were stimulated with fMLP (fMet-Leu-Phe) for the times indicated were incubated with 8ug of P2488-05. The precipitated proteins were separated by SDS-PAGE, transferred to nitrocellulose and probed with R0400 (upper panel) or anti-cdc42 (lower panel)