The purity of recombinant hRANKL was determined by SDS-PAGE of 6ug reduced (+) and non-reduced (-) recombinant hRANKL and staining overnight with Coomassie Blue.
The induction of tartrate resistant acid phosphatase (TRAP) in Raw 264.7 cells was assessed. Raw 264.7 cells were treated with increasing concentrations of hRANKL for 4 days. Cells were lysed and TRAP activity of cell extracts was assessed and OD450 determined.
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