(A) SDS-PAGE of Hla: 1ug (lane 1) and 5ug (lane 2). (B) Western Blot detection of Hla at 5ng, 10ng and 50ng (lanes 1-3), using 299573 (1ug/ml) and an anti-mouse IgG (HRP) conjugate followed by TMB membrane substrate.
Lysis of rabbit red blood cells by S. aureus Hla using 299573. Red blood cell lysis was determined by absorbance at OD 416nm after 30 min incubation at 37°C with Hla.
Supplier Page from United States Biological for Alpha Hemolysin (Hla), Recombinant