Description
alpha-D-mannosidase, p-nitrophenyl-alpha-mannosidase, alpha-D-mannopyranosidase, alpha mannosidase, exo-alpha-mannosidaseAlpha Mannosidase from Jack Bean cleaves α(1-2,3,6)-linked mannose.This enzyme is often used in conjunction with Core Alpha-(1-6) Mannosidase (E-AM02) if a noncleavable core α(1-6) mannose is present on the substrate.SpecifictyAll Alpha-(1-2,3,6)-linked mannosePurityEach lot of α(1-2,3,6) mannosidase is tested for contaminating substances by incubating the enzyme for 24 hours at 37°C with substrates indicated in the table below. No detectable activity is evident for any of these potential contaminants. The detection limit of this assay is 5 µU/mL (IUB).For the protease assay, 10 µg of denatured BSA is incubated for 24 hours with 2 µl of enzyme. Analysis of the BSA band after SDS-PAGE should show no evidence of degradation.Molecular weighttwo polypeptides of 64,000 and 44,000 daltonsSpecific Activity AssayOne unit of Alpha Mannosidase is defined as the amount of enzyme required to hydrolyze 1 µmole of p-nitrophenyl-alpha-p-mannoside to p-nitrophenol in 1 minute at pH 5.0 and 37˚C.ContentsAlpha-(1-2,3,6) Mannosidase in 150 mM sodium phosphate, 0.1 mM ZnCl2 pH 7.5. (pH 7.5).Included with 20 µL and 60 µL pack sizes:200 µl 5x Reaction Buffer 5.0 (250 mM sodium phosphate, pH 5.0).Suggested usage1. Add up to 1 nmol of oligosaccharide.2. Add deionized water to 15 µl.3. Add 4 µl 5x Reaction Buffer 5.0.4. Add 1 µL of enzyme5. Incubate ten minutes at 37˚C