Description
Thermostable T7 RNA polymerase is a genetically engineered T7 RNA polymerase. The enzyme highly specifically recognizes the T7 promoter sequence and can be highly transcribed in vitro at 50 ℃. In the process of transcription in vitro, it can improve the capping efficiency and eliminate the production of dsRNA by-products.Product featuresIt has high specificity for T7 promoter and is used for the synthesis of RNA (including microRNA) in vitro.Product usage1) Synthesis of single stranded RNA;2) Synthesis of highly specific RNA probes;3) Synthetic siRNA precursor;4) Making the precursor of RNA splicing reaction;5) Cap analog was used as primer to make capped mRNA.Usage suggestionsFor efficient transcription of a specific region, it is suggested that the template DNA be pre cut into flat ends or 5 'protruding ends downstream of the region.Spermidine in the buffer may combine with nucleic acid to form insoluble substance. It is suggested to add template DNA finally.Storage temperature-Long term storage at 20 ℃ or -80 ℃.Quality assuranceAfter column purification for many times, only a clear and single target band can be seen by SDS-PAGE gel detection, with a purity of 95%. No E. coli DNA residue, RNase, endonuclease and exonuclease contamination can be detected by PCR.Activity definitionUnder the condition of 50 ℃ and ph8.0, the amount of enzyme required to add 1nmol of [3h] GMP into the acid insoluble precipitate within 1 hour is defined as 1 active unit