Supplier Page from Biorbyt for Recombinant Human Osteopontin/OPN
Biological Activity: Measured by the ability of the immobilized protein to support the adhesion of HEK293 human embryonic kidney cells. When 1 x105 cells/well are added to a rHuOsteopontin/OPN coated plate, cell adhesion is enhanced in a dosedependent manner after 1 hour incubation at 37˚C. The ED50 for this effect is typically 0.1-0.6 µg/mL. rHuThrombin proteolytic treatment of this rHuOsteopontin can increase HEK293 cell adhesion by about 5-fold.