Collagenase, Type 3 LS004182 from Worthington Biochemical Corporation

Product Specs
ItemCollagenase, Type 3
CompanyWorthington Biochemical Corporation
Price $215.00Supplier Page View Company Product Page
Catalog NumberLS004182
Source/Expression SystemClostridium histolyticum
StorageStore at 2-8°C
Activity≥100 units per mg dry weight
Size1 gm

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Worthington Biochemical Corporation

Lakewood, New Jersey 8701

United States

Phone: 1.800.445.9603
1.732.942.1660

Email: custservice@worthington-biochem.com

Company Profile

Website: worthington-biochem.com

(1) Escherichia coli Nissle 1917 alleviates Alzheimer's disease in mice through OmpA-containing outer membrane vesiclesCell Rep MedMay 19, 2026Junhua Xie, Xi-Jian Dai, Qiqiong Li, Wanyu Zhang, Xinke Nie, Haihua Ji, Xinyang Chen, Yuchen Wang, Jing Feng, Zhipeng Li, et al.cocktail,Thermo Fisher Scientific,Cat#: 78442 "Collagenase, Type 3",Worthington Biochemical,Cat#: LS004182 Normal goat serum,Servicebio,Cat#: G1208 DAPI,Beyotime,Cat#: C1006 DAPI,Beyotime,Cat#: 62248 Antifade

(2) Bioengineered iPSC-derived human macrophages with increased angiotensin-converting enzyme (ACE) expression suppress solid tumor growthSignal Transduct Target TherApril 13, 2026Tomohiro Shibata, Shabir Bhat, DuoYao Cao, Suguru Saito, Noor Khan, Ellen A. Bernstein, Takeshi Tokudome, Derick Okwan-Duodu, Warren G. Tourtellotte, Kenneth E. Bernstein, et al.and digested for 30 min in RPMI medium with 1.5 mg/ml collagenase IV (Worthington Biochemical Corp., LS004182) and 0.1 mg/ml DNase I (Promega, M6101) at 37 °C. Collagenase was neutralized with RPMI medium supplemented

(3) ZFP148 is a transcriptional repressor of cytolytic effector CD8+ T cell differentiationNature ImmunologyMarch 27, 2026Tong Xiao, Xingyu Chen, No-Joon Song, Ryan J. Brown, Anjun Ma, Jay K. Mandula, Amir Yousif, Yi Wang, Minh Quynh May Le, Jianying Li, et al.Lungs were minced and digested with Collagenase Type III (Worthington Biochemical Corporation, cat. no. LS004182) for 90 min at 37 °C, passed through 70-μm strainers, and lymphocytes were isolated by Percoll gradient

(4) Unravelling genome-wide mosaic microsatellite mutations at single-cell resolutionbioRxivFebruary 6, 2026Chunyi Wang, Wenxuan Fan, Weixiang Wang, Yonghe Xia, Jinhong Lu, Xiaoyu Ma, Jichuan Yu, Yunchao Zheng, Yan Luo, Wenlong Li, et al.manually minced in 1.5 mL tubes containing collagenase type 3 (300 U/mL) (Worthington Biochemical, #LS004182) and hyaluronidase (100 U/mL) (Worthington Biochemical, #LS002592) for 2 hours, with gentle pipetting

(5) Microbiota utilization of intestinal amino acids modulates cancer progression and anticancer immunityCell Host & MicrobeJanuary 14, 2026Shanshan Qiao, Ting-Ting Li, Mingeum Jeong, Chuanfa Liu, Sayaka Mizutani, Sung-Min Hwang, Yaxin Li, Mengze Lyu, Kazuhiro Nishiyama, Yu-Ang Tang, et al.21985023 Collagenase D,Sigma-Aldrich,Cat# 11088882001 Collagenase Type 3,Worthington Biochemical,Cat # LS004182 DNase I,Zymo Research,Cat # E1011 Percoll,Cytiva,Cat # 17-0891-01 RPMI 1640 medium,Corning,Cat #

(6) The gain-of-function TREM2-T96K mutation increases risk for Alzheimer’s disease by impairing microglial functionNeuronJanuary 7, 2026Dominika J. Pilat, Hoang Le, Dmitry Prokopenko, Chih-Chung Jerry Jerry Lin, William A. Eimer, Luisa Quinti, Evan P. Gavrilles, Sheyla N. Garcia, Sara N. Heitman, Alzheimer’s Disease Neuroimaging Initiative (ADNI), et al.Dispase (2 U/ml, LS02104; Worthington Biochemical Corporation) and Collagenase Type 3 (200 U/ml, # LS004182; Worthington Biochemical Corporation). Microglia were isolated according to our previous protocol.28

(7) Single-cell transcriptomics reveals notch regulation in quiescent LEPR⁺ endometrial mesenchymal stem cellsStem Cell Res TherDecember 23, 2025Yuan Fang, Dandan Cao, Cheuk-Lun Lee, Philip C. N. Chiu, Ernest H. Y. Ng, William S. B. Yeung, Rachel W. S. Chansaline (PBS) containing collagenase type III (0.3 mg/ml, Worthington Biochemical Corporation, USA, #LS004182) and deoxyribonuclease type I (40 μg/ml, Worthington Biochemical Corporation, #LS002139) in a shaking

(8) High expression of interleukin-18 receptor alpha correlates with severe respiratory viral disease and defines T cells with reduced cytotoxic signaturesNature CommunicationsNovember 24, 2025Aira F. Cabug, Jeremy Chase Crawford, Hayley A. McQuilten, Isabelle J. H. Foo, Lilith F. Allen, Deborah Gebregzabher, Robert C. Mettelman, Tanya Novak, Janet Chou, Louise C. Rowntree, et al.digested in collagenase III (2 mg/mL Collagenase Type III, Worthington Biochemical Corporation, Cat # LS004182) and DNAse I (10 μg/mL DNAse I, Sigma Aldrich, Cat # 10104159001) at 37 °C for 40 mins before processing,

(9) Tabula Sapiens reveals transcription factor expression, senescence effects, and sex-specific features in cell types from 28 human organs and tissuesbioRxivSeptember 13, 2025The Tabula Sapiens Consortium, Stephen R QuakeMedChem Express HY-10583) containing Collagenase III (300 U/mL: Worthington Biochemical Corporation LS004182) and DNase I (200 U/mL: Worthington Biochemical Corporation LS002139) at 37°C for 90 minutes. During

(10) Mutations in MLL3 promote breast cancer progression via HIF1α-dependent intratumoral recruitment and differentiation of regulatory T cellsImmunityAugust 12, 2025Boutet M, Nishitani K, Couturier N, Erler P, Zhang Z, Militello AM, Coutinho De Miranda M, Barbieux E, Guillen E, Leavenworth JW, et al.organoid Mammary glands were minced and digested with 300 units/ml collagenase type 3 (Worthington Biochemical, LS004182), and 10 μg/ml Dnase I (Worthington Biochemical, LS002139) in the DMEM/F-12 medium at 37°C for 2 hrs

(11) Engineered Endosymbionts that Modulate Primary Macrophage Function and Attenuate Tumor Growth by Shifting the Tumor MicroenvironmentACS Applied Bio MaterialsJuly 21, 2025Cody S. Madsen, Ashley V. Makela, Chima V. Maduka, Emily M. Greeson, Anthony Tundo, Evran Ural, Satyajit Hari Kulkarni, Ahmed A. Zarea, Matti Kiupel, Maryam Sayadi, et al.digestion using a solution containing DMEM, collagenase III (300 U/ml; Worthington Biochemical, cat. no. LS004182) and DNase I (100 U/ml; Worthington Biochemical, cat#LS002139). Tumors were digested for 90 min at 37

(12) Piezo1 deletion enhances cross-priming of CD8+ T cells by tumor-infiltrating CD11b+ dendritic cellsJ Immunother CancerJune 23, 2025Melissa Bonner, David Askew, Vrishabhadev Sathish Kumar, Suzanne L Tomchuck, Saada Eid, Muta Abiff, Jay T Myers, Phuong Nguyen, Justin W A Garyu, Tyler E Miller, et al.then suspended in tumor digestion buffer (RPMI-1640, 1 mg/mL Collagenase D (Worthington Biochemical LS004182), 20 U /mL DNase I (Roche 04716728001)) and incubated in a 37°C shaker at 735 RPM for 45–60 minutes

(13) Regulation of axonal microtubule polarity orientation in different kinds of neuronsFASEB JJune 15, 2025Ankita Patil, Shrobona Guha, Ilgin Isiltan, Hemalatha Muralidharan, Kirankumar Madugula, Peter W. BaasThe SCGs were dissected and enzymatically digested with 0.25% collagenase (Worthington Biochemical #LS004182) for 15 min, followed by 0.25% trypsin for 45 min, also as previously described (He and Baas, 2003;

(14) Regulation of Axonal Microtubule Polarity Orientation in Different Kinds of NeuronsThe FASEB JournalJune 15, 2025Ankita Patil, Shrobona Guha, Ilgin Isiltan, Hemalatha Muralidharan, Kirankumar Madugula, Peter W. BaasThe SCGs were dissected and enzymatically digested with 0.25% collagenase (Worthington Biochemical #LS004182) for 15 min, followed by 0.25% trypsin for 45 min, also as previously described (He and Baas, 2003;

(15) Saponin-based adjuvant uptake and induction of antigen cross-presentation by CD11b+ dendritic cells and macrophagesNPJ vaccinesJanuary 22, 2025Huis In 't Veld LG, Cornelissen LA, van den Bogaard L, Ansems M, Ho NI, Adema GJ.dissociated using needles and then incubated in RPMI medium containing 1 mg/ml collagenase type III (LS004182, Worthington Biochemical) and 30 µg/ml DNAse type I (11284932001, Roche) for 30 min at 37 °C. Lymph node

(16) Sensory nerve release of CGRP increases tumor growth in HNSCC by suppressing TILsMedMarch 8, 2024Laurel B. Darragh, Alexander Nguyen, Tiffany T. Pham, Shaquia Idlett-Ali, Michael W. Knitz, Jacob Gadwa, Sanjana Bukkapatnam, Sophia Corbo, Nicholas A. Olimpo, Diemmy Nguyen, et al.,Worthington Biochemical Corporation,Cat# LS003571 Collagenase Type III,Worthington Biochemical Corporation,Cat# LS004182 eBioscience 1X RBC Lysis Buffer,Invitrogen,Ref# 00-4333-57 eBioscience FOXP3/Transcription Factor

(17) Unveiling Epigenetic Vulnerabilities in Triple-Negative Breast Cancer through 3D Organoid Drug ScreeningPharmaceuticals (Basel, Switzerland)February 8, 2024Rao X, Qiao Z, Yang Y, Deng Y, Zhang Z, Yu X, Guo X.viscous. We modified the enzymatic digestion process by utilizing 1.5 mg/mL collagenase type III (#LS004182; Worthington Biochemical Corporation, Worthington, OH, USA) instead of Collagenase Type II. Collagenase

(18) Nε-Carboxymethyl-Lysine Modification of Extracellular Matrix Proteins Augments Fibroblast ActivationInternational Journal of Molecular SciencesOctober 31, 2023Harshavardhana H. Ediga, Patrick Hester, Adithi Yepuri, Geereddy Bhanuprakash Reddy, Satish K. Madalaand digested at 37 °C for 60 min in 5 mL of DMEM or IMDM containing collagenase type 3 (2 mg/mL; Cat: LS004182; Worthington Biochemical Corporation, Lakewood NJ, USA) for human or mouse lungs, respectively. Digested

(19) Clec4A4 Acts as a Negative Immune Checkpoint Regulator to Suppress Antitumor ImmunityCancer immunology researchSeptember 1, 2023Uto T, Fukaya T, Mitoma S, Nishikawa Y, Tominaga M, Choijookhuu N, Hishikawa Y, Sato K.refs. 8, 19, 20, 26), tissue samples were digested with collagenase type III (Worthington Biochemical, LS004182) at 37°C for 20 minutes, and were ground between glass slides. Splenocytes were treated with Red Blood Cell Lysing Buffer Hybri-Max

(20) Dantrolene inhibits lysophosphatidylcholine-induced valve interstitial cell calcific nodule formation via blockade of the ryanodine receptorFrontiers in cardiovascular medicineJanuary 1, 2023Sylvester CB, Amirkhosravi F, Bortoletto AS, West WJ 3rd, Connell JP, Grande-Allen KJ.valves were minced and incubated in a mixture of 300 U/ml collagenase type III (Worthington Biochemical LS004182), 50 U/ml hyaluronidase, and 0.5 U/ml neutral protease (Stemcell Technologies 07913) in DMEM with ABAM

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Collagenase, Type 3 from Worthington Biochemical Corporation

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Worthington Biochemical Corporation

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Collagenase, Type 3 from Worthington Biochemical Corporation

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