Elastase, Lyophilized LS002294 from Worthington Biochemical Corporation

Product Specs
ItemElastase, Lyophilized
CompanyWorthington Biochemical Corporation
Price $1,125.00Supplier Page View Company Product Page
Catalog NumberLS002294
Source/Expression SystemPorcine Pancreas
StorageStore at 2-8°C
Activity≥3 units per mg protein
Size1 gm

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Worthington Biochemical Corporation

Lakewood, New Jersey 8701

United States

Phone: 1.800.445.9603
1.732.942.1660

Email: custservice@worthington-biochem.com

Company Profile

Website: worthington-biochem.com

(1) Transcriptional and spatial profiling of fibroblasts from human lungs highlights CTHRC1+ cells as fibrogenic signaling hubs in fibrosisbioRxivApril 10, 2026N.D. Vanegas-Avendano, H. Chen, J. Wellmerling, J. Rodriguez-Lopez, A. Ghobashi, V. Peters, C. Sen, B. F. Reader, K. Shilo, B. Gomperts, et al.included collagenase type IV (Worthington Biochemical, LS004186), elastase (Worthington Biochemical, LS002292), dispase (StemCell, 7913), and DNase I (Worthington Biochemical, LS002139). After dissociation, 1X

(2) Hh and EGFR-Ras signaling promote distinct steps of tumor progression in the Drosophila follicle epitheliumNat CommunMarch 24, 2026Sari Anschütz, Hannah Müller, Andrea Schubert, Jobelle M. Peralta, Todd G. Nystul, Katja Rust13141014) and dissociated in Cell Dissociation Buffer with 4 mg/mL elastase (Worthington Biochemical LS002292) and 2.5 mg/mL collagenase (Invitrogen # 17018-029) for 20 min at RT under constant pipetting to promote

(3) A new mouse model of biomass smoke-related chronic obstructive pulmonary disease combining porcine pancreatic elastase nebulizationJournal of Thoracic DiseaseDecember 31, 2025Jiaqi Lin, Qiumeng Li, Yating Chen, Jianxiong Lai, Yiqi Lin, Dongxing Zhaonebulizer (PARI BOY; PARI GmbH, Starnberg, Germany) was used to deliver porcine pancreatic elastase (LS002292; Worthington Biochemical Corporation, Lakewood, NJ, USA). The dose was 6 U per mouse, nebulized for

(4) circ_0103896/miR-432–5p/FTO feedback loop suppresses the formation and progression of intracranial aneurysmNoncoding RNA ResDecember 15, 2025Zhiwen Lu, Shijie Zhu, Yina Wu, Fengfeng Xu, Qinghai Huangfor 45 min in 100 μL of DMEM supplemented with collagenase type I (6 mg/mL) and elastase (2.5 mg/mL; LS002290, Worthington Biochemical). After enzymatic digestion, the dissociated cells were seeded and cultured

(5) Non-Ser36 phosphorylated p66Shc inhibits TRPC-dependent and TRPC-independent calcium influx in vascular smooth muscle cellsBiochemical and Biophysical Research CommunicationsNovember 1, 2025Perrin Schupbach, Bradley Miller, Andrey SorokinSerum Albumin (BSA, Cat #A8412) - Sigma-Aldrich (St. Louis, MO); Papain (Cat #LK003176), Elastase (Cat #LS002290), Collagenase Type 2 (Cat #LS004174), and Soybean Trypsin Inhibitor (Cat #LS003570) - Worthington Biochemical Corporation

(6) Interplay of ECM organization, ROCK signaling, and cell polarity drives mesothelium formation and lung growthNat CommunOctober 30, 2025Xinyuan Liu, Bingying Lin, Peiyi Li, Zhiqiang Cai, Weitao Cao, Weiping Yang, Jiahang Zeng, Le Li, Yumin Zhou, Danping Huang, et al.lungs were isolated from E16.5 embryos and cultured using an established protocol83. For elastase (LS002290, Worthington Biochemical Corporation) treatment, a 2 U/mL stock solution was diluted to 2 mU/mL. For

(7) Protocol for establishing genetically engineered murine lung organoids mimicking cell plasticity and regenerationSTAR ProtocolsSeptember 1, 2025Bongjun Kim, Jae-Il Park15630080 Collagenase type I,Worthington Biochemical,Cat# CLS-1 LS004197 Elastase,Worthington Biochemical,Cat# ESL LS002294 DNase I,Worthington Biochemical,Cat# D LS002007 Bovine gelatin,Sigma,Cat# G1393 EDTA solution,Sigma,Cat#

(8) miR-190 is a Key Regulator in Establishing Cell Polarity and Specification in the Drosophila Nervous SystembioRxivMay 30, 2025Gerson Ascencio, Laura Galvan, Julie M. Sanchez, Ariana Nagainis, Cynnie Tam, Lauren M. Goins, Blake Riggs1mL of digestion buffer was added. Digestion buffer included elastase (4mg/ml Worthington Biochemical LS002292), collagenase (2.5 mg/ml Sigma-Aldrich C9722), and cell dissociation buffer (1mL Thermo Fisher Scientific

(9) A searchable atlas of pathogen-sensitive lncRNA networks in human macrophagesNat CommunMay 21, 2025Nils Schmerer, Harshavardhan Janga, Michelle Aillaud, Janina Hoffmann, Marina Aznaourova, Sarah Wende, Henrike Steding, Luke D. Halder, Michael Uhl, Fabian Boldt, et al.was digested in 40 ml BSSB containing 2.86 mg trypsin (#T4799, Sigma) and 300 µl of 5 U/ml elastase (#LS002292, Worthington Biochemical) at 37 °C for 45 min while shaking. Digestion was stopped by transferring the

(10) Functions for platelet factor 4 (PF4/CXCL4) and its receptors in fibroblast-myofibroblast transition and fibrotic failure of arteriovenous fistulas (AVFs)The Journal of Vascular AccessNovember 1, 2024Yuxuan Xiao, Laisel Martinez, Zachary Zigmond, Daniel Woltmann, Diane V Singer, Harold A Singer, Roberto I Vazquez-Padron, Loay H SalmanLakewood, NJ), 1 mg/ml soybean trypsin inhibitor (LS003571, Worthington), 0.744 units/ml elastase (LS002292, Worthington) in wash buffer for 8–10 min and placed back in fresh wash buffer. The adventitial layer

(11) Matrix stiffness regulates the triad communication of adipocytes/macrophages/endothelial cells through CXCL13Journal of Lipid ResearchSeptember 1, 2024Arthur Choisez, Seiichiro Ishihara, Takuro Ishii, Yidan Xu, Sepideh D. Firouzjah, Hisashi Haga, Ryoichi Nagatomi, Joji Kusuyamaand pH 7.4) containing 0.5 mg/ml dispase (LS02110, Worthington Biochemical) and 0.5 mg/ml elastase (LS002292, Worthington-Biochemical) at 37°C for 1 h with gentle agitation by inversion every 10–15 min. Subsequently,

(12) NAD(+) metabolism enzyme NNMT in cancer-associated fibroblasts drives tumor progression and resistance to immunotherapy by modulating macrophages in urothelial bladder cancerJournal for immunotherapy of cancerJuly 27, 2024Yang M, Wang B, Hou W, Zeng H, He W, Zhang XK, Yan D, Yu H, Huang L, Pei L, et al.deoxyribonuclease l (LS002138, 0.025 mg/mL, Worthington Biochemical Corporation, USA), and elastase (LS002290, 0.025 mg/mL, Worthington Biochemical Corporation, Lakewood, New Jersey, USA). The digested substance

(13) A porcine model of thoracic aortic aneurysms created with a retrievable drug infusion stent graft mirrors human aneurysm pathophysiologyJVS-Vascular ScienceJuly 2, 2024Dahlia M. Kenawy, Jordan F. Stafford, Foued Amari, Drayson Campbell, Mahmoud Abdel-Rasoul, Jennifer Leight, Youngjae Chun, Bryan W. Tillman2.5% trypsin-EDTA (Ref 25200-056, Gibco, Thermo Fisher Scientific, Waltham, MA), 500 U elastase (Cat# LS002294, Worthington Biochemical Corporation, Lakewood, NJ), and 8000 U collagenase type 1 (Cat# LS004196, Worthington Biochemical Corporation

(14) Intense impact of IL-1β expressing inflammatory macrophages in acute aortic dissectionScientific reportsJune 28, 2024Inoue T, Emoto T, Yamanaka K, Chomei S, Miyahara S, Takahashi H, Shinohara R, Kondo T, Taniguchi M, Furuyashiki T, et al.soybean trypsin inhibitor (LS003571, Worthington Biochemical Corp), 0.1875 mg/ml elastase lyophilized (LS002292, Worthington Biochemical Corp), 0.24 mg/ml hyaluronidase type I (H3506, Sigma), and 2.38 mg/ml 4-(2-hydroxyethyl)-1-piperazineethanesulfonic

(15) Inhibition of smooth muscle cell death by Angiotensin 1-7 protects against abdominal aortic aneurysmBioscience ReportsNovember 30, 2023Anshul S. Jadli, Karina P. Gomes, Noura N. Ballasy, Tishani Methsala Wijesuriya, Darrell Belke, Paul W.M. Fedak, Vaibhav B. Patelsolution containing 1 mg/ml collagenase II (#LS004174, Worthington Biochemical) and 1 mg/ml elastase (#LS002292, Worthington Biochemical) at 37°C in a 5% CO2 incubator for 10 min. The adventitial and inner layers

(16) The formation of KV2.1 macro-clusters is required for sex-specific differences in L-type CaV1.2 clustering and function in arterial myocytesCommunications BiologyNovember 14, 2023Collin Matsumoto, Samantha C. O’Dwyer, Declan Manning, Gonzalo Hernandez-Hernandez, Paula Rhana, Zhihui Fong, Daisuke Sato, Colleen E. Clancy, Nicholas C. Vierra, James S. Trimmer, et al.dissecting solution supplemented with 1.6 mg/ml collagenase H, 0.5 mg/ml elastase (Worthington Biochemical, LS002292), and 1 mg/ml trypsin inhibitor from Glycine max at 37 °C. Arteries were rinsed three times with dissection

(17) Mural cell-derived chemokines provide a protective niche to safeguard vascular macrophages and limit chronic inflammationImmunityOctober 10, 2023Pekayvaz K, Gold C, Hoseinpour P, Engel A, Martinez-Navarro A, Eivers L, Coletti R, Joppich M, Dionísio F, Kaiser R, et al.containing collagenase type 2 (Worthington Biochemical® LS004174) and elastase (Worthington Biochemical® LS002292) in PBS containing Mg2+ and Ca2+ for 10 min at 37 °C in a thermo shaker with 400 rpm. Aortas were transferred

(18) The proton channel OTOP1 is a sensor for the taste of ammonium chlorideNature CommunicationsOctober 5, 2023Ziyu Liang, Courtney E. Wilson, Bochuan Teng, Sue C. Kinnamon, Emily R. Limanmix consisting of Tyrode’s solution supplemented with 1 mg/mL elastase (Worthington Biochemical, Cat# LS002290), and 2.5 mg/mL Dispase II (Sigma Aldrich, Cat# D4693), was injected between the epithelium and muscle

(19) The effect of Toll-like receptor agonists on the immunogenicity of MVA-SARS-2-S vaccine after intranasal administration in miceFrontiers in Cellular and Infection MicrobiologyOctober 3, 2023Kim Thi Hoang Do, Stefanie Willenzon, Jasmin Ristenpart, Anika Janssen, Asisa Volz, Gerd Sutter, Reinhold Förster, Berislav Bošnjakanalysis. Right lung lobes were next inflated with #1 digestion media made of 4 U/ml of Elastase (cat.# LS002292, Worthington Biochemical Corporation), 1 U/ml of Dispase II (cat.# 4942078001, Sigma-Aldrich), 200 µg/ml

(20) Determination of Interleukin-17A and Interferon-γ Production in γδ, CD4(+), and CD8(+) T Cells Isolated from Murine Lymphoid Organs, Perivascular Adipose Tissue, Kidney, and LungBio-protocolMay 20, 2023Comeau K, Caillon A, Paradis P, Schiffrin EL.catalog number: LS004176)Elastase, lyophilized, ≥3 U/mg protein (Worthington Biochemical, catalog number: LS002292)Hyaluronidase, lyophilized, ≥300 U/mg protein (Sigma-Aldrich, catalog number: H2126-500MG)Trypsin inhibitor, soybean, purified

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Elastase, Lyophilized from Worthington Biochemical Corporation

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Worthington Biochemical Corporation

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Elastase, Lyophilized from Worthington Biochemical Corporation

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