Collagenase, Type 2 LS004174 from Worthington Biochemical Corporation

Product Specs
ItemCollagenase, Type 2
CompanyWorthington Biochemical Corporation
Price $42.00Supplier Page View Company Product Page
Catalog NumberLS004174
Source/Expression SystemClostridium histolyticum
StorageStore at 2-8°C
Activity≥125 units per mg dry weight
Size100 mg

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Worthington Biochemical Corporation

Lakewood, New Jersey 8701

United States

Phone: 1.800.445.9603
1.732.942.1660

Email: custservice@worthington-biochem.com

Company Profile

Website: worthington-biochem.com

(1) Targeting TPX2-dependent lineage plasticity by CDK4/6 inhibition reverses therapy resistance in neuroendocrine bladder carcinomaCell Reports MedicineApril 21, 2026Zehua Chen, Shengmeng Peng, Shi Fu, Shizhi Lin, Yiwen Su, Xinxin He, Yangjie Zhang, Junlin Lu, Sen Liu, Hengji Zhan, et al.enzymatically digested with collagenase at 37°C for 30 min (Worthington Biochemical Corporation, Cat#LS004174).54 The digestion was terminated by adding 10% FBS, and the resulting suspension was filtered through

(2) Sympathetic nerve–fibroblast crosstalk drives nerve injury, fibroblast activation, and matrix remodeling in pancreatic cancerJCI InsightApril 8, 2026Ariana L. Sattler, Parham Diba, Kevin Hawthorne, Carl Pelz, Joe Grieco, Tetiana Korzun, Bryan Chong, M.J. Kuykendall, Rosalie C. Sears, Daniel L. Marks, et al.was removed from the ganglia. SCGs were dissociated with collagenase type 2 (Worthington Biochemical, LS004174) for 30–35 minutes at 37°C and inverted every 10 minutes, then washed in DMEM (Gibco, 11965-092) containing

(3) USP13-Mediated selective autophagy of eIF5B promotes pathological cardiac hypertrophyCellular and Molecular Life SciencesDecember 16, 2025Yutong Ye, Juan Xu, Chenyang Jin, XinTao Li, Xiaoyu Wu, Changzuan Zhou, Longzhe Gao, Zikan Zhong, Xudong Li, Songwen Chen, et al.25200056) at 4 °C overnight, followed by digestion in 1 mg/ml collagenase II (Worthington Biochemical, LS004174) and 5 mg/ml bovine serum albumin (BSA, Sigma-Aldrich, V900933) the next day. Cells were harvested by

(4) Spatiotemporal gene expression and cellular dynamics of the developing human heartNature GeneticsNovember 1, 2025Enikő Lázár, Raphaël Mauron, Žaneta Andrusivová, Julia Foyer, Mengxiao He, Ludvig Larsson, Nick Shakari, Sergio Marco Salas, Christophe Avenel, Sanem Sariyar, et al.Falcon tube containing an enzymatic solution of Collagenase II (200 U ml−1; Worthington Biochemical, LS004174), DNase I (1 mg ml−1; Worthington Biochemical, LK003170) in Earle’s balanced salt solution (EBSS; Worthington Biochemical

(5) Non-Ser36 phosphorylated p66Shc inhibits TRPC-dependent and TRPC-independent calcium influx in vascular smooth muscle cellsBiochemical and Biophysical Research CommunicationsNovember 1, 2025Perrin Schupbach, Bradley Miller, Andrey SorokinSigma-Aldrich (St. Louis, MO); Papain (Cat #LK003176), Elastase (Cat #LS002290), Collagenase Type 2 (Cat #LS004174), and Soybean Trypsin Inhibitor (Cat #LS003570) - Worthington Biochemical Corporation (Lakewood, NJ);

(6) Porphyromonas gingivalis Type 9 Secretion System Promotes Dysregulation of Vascular Smooth Muscle Cell Plasticity With Perturbed TGF-β/Smad SignalingMicrobiologyopenOctober 22, 2025Priscilla L. Phillips, Leticia Reyesmethod (Villa‐Bellosta and Hamczyk 2015). Collagenase type 2 (Worthington Biochemical Corp., Catalog # LS004174) was used for tissue dissociation. For all experiments, AoSMC was initially prepared by culturing in

(7) Glutamate receptor-T cell receptor signaling potentiates full CD8+ T cell activation and effector function in tumor immunityiScienceOctober 17, 2025Maria Teresa P. Teresa P de Aquino, Thomas W. Hodo, Salvador González Ochoa, Roman V. Uzhachenko, Muna A. Mohammed, J. Shawn Goodwin, Thanigaivelan Kanagasabai, Alla V. Ivanova, Anil ShankerP36965 Poly-D-Lysine,Thermo fisher,Cat# A3890401 Collagenase type 2,Worthington Biochemical,Cat# LS004174 SRB Assay / Sulforhodamine B Assay Kit,Abcam,Cat# ab235935 IL-2/Fc chimera mouse recombinant,SIGMA,Cat#

(8) Sirtuin 1 Activation Mitigates Murine Vasculitis Severity by Promoting Autophagy and MitophagybioRxivSeptember 11, 2025Asli E. Atici, Prasant K. Jena, Thacyana T. Carvalho, Benjamin L. Ross, Emily A. Aubuchon, Malcolm E. Lane, Angela C. Gomez, Youngho Lee, Shuang Chen, Timothy R. Crother, et al.minutes in enzyme solution 1 at 37 °C and 5% CO2 (1 mg/ml collagenase type II (Worthington Biochemical; LS004174), 0.744 units/ml elastase (Worthington Biochemical; LS0022792), and 1% P/S). Adventitia was stripped

(9) EBBP‐Mediated Integrated Stress Response Attenuates Anthracycline‐Induced Cardiotoxicity by Inhibiting the Ferroptosis of CardiomyocytesAdvanced ScienceAugust 1, 2025Zilong Chen, Can Chen, Yichen Wu, Yinxue Xia, Ruijie Luo, Jiangcheng Shu, Long Chen, Zhaohui Wang, Cheng Wang, Kai Huang1–3) were excised, minced, and subsequently subjected to digestion using 0.1% type II collagenase (#LS004174, Worthington Biochemical, USA) at 37 °C. The resulting digestion solution containing free cells, was

(10) Activating nuclear receptor subfamily 2 group F member 2 in adipocyte stem cells rescues beige adipocyte metabolism impaired by excess early-life omega-6 fatty acidsClinical NutritionAugust 1, 2025Snehasis Das, Rohan R. Varshney, Jacob W. Farriester, Gertrude Kyere-Davies, Alexandrea E. Martinez, Kaitlyn B. Hill, Michael Kinter, Gregory P. Mullen, Prabhakara R. Nagareddy, Michael C. RudolphSolution (HBSS) (Sigma, H8264) containing 3% BSA, 0.8 mg/ml collagenase type 2 (Worthington Biochemical, LS004174), 0.8 mM ZnCl2, 2.5 mM glucose and 0.2 μM adenosine for 45 minutes at 37°C in an orbital shaker at 150

(11) Semaphorin7A and PD-L1 cooperatively drive immunosuppression during mammary involution and breast cancerCell ReportsMay 27, 2025Alan M. Elder, Heather R. Fairchild, Kelsey T. Kines, Lauren M. Cozzens, Alexandria R. Becks, Jill E. Slansky, Steven M. Anderson, Traci R. Lyonsbuffer consisting of a final concentration of 500units/ml collagenase II (Worthington Biochemical; Cat# LS004174), 500units/ml collagenase IV (Worthington Biochemical; Cat# LS004186), and 20 mg/ml DNase I (Worthington Biochemical

(12) Dietary oleic acid drives obesogenic adipogenesis via modulation of LXRα signalingCell ReportsApril 22, 2025Allison Wing, Elise Jeffery, Christopher D. Church, Jennifer Goodell, Rocío del M. Saavedra-Peña, Moumita Saha, Brandon Holtrup, Maud Voisin, N. Sima Alavi, Mariana Floody, et al.Bovine serum albumin,AmericanBio,AB01088 HBSS,Gibco,14185–052 Collagenase Type II,Worthington Biochemical,LS004174 Phosflow Lyse/Fix Buffer,BD Biosciences,558049 DNase I,Worthington Biochemical,LS002007 Intracellular

(13) Lineage Tracing and Single-Cell RNA Sequencing Reveal a Common Transcriptional State in Breast Cancer Tumor-Initiating Cells Characterized by IFN/STAT1 ActivityCancer ResApril 15, 2025Eric P. Souto, Ping Gong, John D. Landua, Ramakrishnan Rajaram Srinivasan, Abhinaya Ganesan, Lacey E. Dobrolecki, Stephen C. Purdy, Xingxin Pan, Michael Zeosky, Anna Chung, et al.(Worthington Biochemical Corporation, cat. #LS004176), 250 U/mL Collagenase II (Worthington Biochemical Corporation, cat. #LS004174), and 2 μg/mL DNase I (STEMCELL Technologies, cat. #07900)] per 1 g tumor at 37°C in a gentleMACS Dissociator

(14) Lineage Tracing and Single-Cell RNA Sequencing Reveal a Common Transcriptional State in Breast Cancer Tumor–Initiating Cells Characterized by IFN/STAT1 ActivityCancer ResFebruary 11, 2025Eric P. Souto, Ping Gong, John D. Landua, Ramakrishnan Rajaram Srinivasan, Abhinaya Ganesan, Lacey E. Dobrolecki, Stephen C. Purdy, Xingxin Pan, Michael Zeosky, Anna Chung, et al.(Worthington Biochemical Corporation, cat. #LS004176), 250 U/mL Collagenase II (Worthington Biochemical Corporation, cat. #LS004174), and 2 μg/mL DNase I (STEMCELL Technologies, cat. #07900)] per 1 g tumor at 37°C in a gentleMACS Dissociator

(15) Brucella abortus impairs T lymphocyte responsiveness by mobilizing IL-1RA-secreting omental neutrophilsNature communicationsJanuary 20, 2025Pellegrini JM, González-Espinoza G, Shayan RR, Hysenaj L, Rouma T, Arce-Gorvel V, Lelouard H, Popoff D, Zhao Y, Hanniffy S, et al.at 37 °C with agitation in RPMI, 2 µg/mL collagenase type II (Worthington Biochemical Corporation, LS004174), 0.2 µg/mL DNAse I (Sigma, DN25100MG). Digestion was stopped in RPMI, 10 mM EDTA, and 2% Bovine Serum

(16) Germline deletion of Rgs2 and/or Rgs5 in male mice does not exacerbate left ventricular remodeling induced by subchronic isoproterenol infusionPhysiol RepJanuary 2, 2025Shelby Dahlen, Ipsita Mohanty, Bo Sun, Sanjana Nallapaneni, Patrick Osei-Owusuand stored at 4 °C, up to 48 h. Collagenase buffer containing 0.5 mg/mL collagenase type 2 (cat. # LS004174, Worthington Biochemical Corp., Lakewood, NJ), 0.5 mg/mL collagenase type 4 (Worthington Biochemical Corp

(17) Functions for platelet factor 4 (PF4/CXCL4) and its receptors in fibroblast-myofibroblast transition and fibrotic failure of arteriovenous fistulas (AVFs)The Journal of Vascular AccessNovember 1, 2024Yuxuan Xiao, Laisel Martinez, Zachary Zigmond, Daniel Woltmann, Diane V Singer, Harold A Singer, Roberto I Vazquez-Padron, Loay H Salmancold wash buffer, and veins were then incubated in an enzyme solution contains 1 mg/ml collagenase II (LS004174, Worthington Biochemical, Lakewood, NJ), 1 mg/ml soybean trypsin inhibitor (LS003571, Worthington),

(18) Distinct muscle regenerative capacity of human induced pluripotent stem cell-derived mesenchymal stromal cells in Ullrich congenital muscular dystrophy model miceStem Cell Research & TherapyOctober 7, 2024Megumi Yokomizo-Goto, Nana Takenaka-Ninagawa, Chengzhu Zhao, Clémence Kiho Bourgeois Yoshioka, Mayuho Miki, Souta Motoike, Yoshiko Inada, Denise Zujur, William Theoputra, Yonghui Jin, et al.the lower and upper arms were collected and digested into single cells using 0.2% collagenase type 2 (LS004174, Worthington Biochemical, NJ, USA) and DMEM (08488–55, Nacalai Tesque, Kyoto, Japan). Subsequently,

(19) PAI-1 Regulates the Cytoskeleton and Intrinsic Stiffness of Vascular Smooth Muscle CellsArteriosclerosis, thrombosis, and vascular biologyOctober 1, 2024Khoukaz HB, Vadali M, Schoenherr A, Ramirez-Perez FI, Morales-Quinones M, Sun Z, Fujie S, Foote CA, Lyu Z, Zeng S, et al.mg/mL), and porcine pancreatic elastase (0.75 units/mL) from Worthington Biochemical, Lakewood, NJ, USA (#LS004174, #LS003570, and #LS002279, respectively). After removing the adventitia, SMCs were isolated and cultured

(20) NR2F2 Reactivation in Early-life Adipocyte Stem-like Cells Rescues Adipocyte Mitochondrial OxidationbioRxivSeptember 12, 2024Snehasis Das, Rohan Varshney, Jacob W. Farriester, Gertrude Kyere-Davies, Alexandrea E. Martinez, Kaitlyn Hill, Michael Kinter, Gregory P. Mullen, Prabhakara R. Nagareddy, Michael C. RudolphSolution (HBSS) (Sigma, H8264) containing 3% BSA, 0.8 mg ml−1 collagenase type 2 (Worthington Biochemical, LS004174), 0.8 mM ZnCl2, 2.5 mM glucose and 0.2 μM adenosine for 45 minutes at 37°C in an orbital shaker at 150

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Collagenase, Type 2 from Worthington Biochemical Corporation

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Worthington Biochemical Corporation

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Collagenase, Type 2 from Worthington Biochemical Corporation

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