Collagenase, Type 3 LS004183 from Worthington Biochemical Corporation

Product Specs
ItemCollagenase, Type 3
CompanyWorthington Biochemical Corporation
Price $900.00Supplier Page View Company Product Page
Catalog NumberLS004183
Source/Expression SystemClostridium histolyticum
StorageStore at 2-8°C
Activity≥100 units per mg dry weight
Size5 gm

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Worthington Biochemical Corporation

Lakewood, New Jersey 8701

United States

Phone: 1.800.445.9603
1.732.942.1660

Email: custservice@worthington-biochem.com

Company Profile

Website: worthington-biochem.com

(1) Structural evidence that a lipid plug controls K2P6.1(TWIK-2) functionNature CommunicationsJune 16, 2026Abhisek Mondal, Sangeeta Niranjan, Daniel L. MinorUCSF IACUC Protocol AN193390 and digested using collagenase (Worthington Biochemical Corporation, #LS004183, 0.8–1.0 mg mL−1) in Ca2+-free ND96 (96 mM NaCl, 2 mM KCl, 3.8 mM MgCl2, 5 mM HEPES pH 7.4). Prior to

(2) Gut microbiota promotes immune tolerance at the maternal-fetal interfaceCellJanuary 8, 2026Julia A. Brown, Mohammed Amir, Shui Yu, Daniel S.H. Wong, Jinghua Gu, Uthra Balaji, Christopher N. Parkhurst, Seunghee Hong, Lucy R. Hart, Hannah C. Carrow, et al.DN25), 800 μg/mL dispase (Gibco 17105–041), and 0.8mg/mL collagenase type 3 (Worthington Biochemical LS004183). Digested tissues were pushed through a 70μm filter to create a single-cell suspension. For placenta,

(3) Structure of the human K2P13.1 channel reveals a hydrophilic pore restriction and lipid cofactor siteNature Structural & Molecular BiologyJuly 1, 2025Shatabdi Roy-Chowdhury, Seil Jang, Fayal Abderemane-Ali, Fiona Naughton, Michael Grabe, Daniel L. MinorCommittee (protocol AN193390) and digested using collagenase (Worthington Biochemical Corporation, LS004183; 0.8–1.0 mg ml−1) in Ca2+-free ND96 (96 mM NaCl, 2 mM KCl, 3.8 mM MgCl2 and 5 mM HEPES pH 7.4). Oocytes

(4) A lipid plug affects K 2P 6.1(TWIK-2) functionbioRxivJune 18, 2025Abhisek Mondal, Sangeeta Niranjan, Daniel L. MinorUCSF IACUC Protocol AN193390 and digested using collagenase (Worthington Biochemical Corporation, #LS004183, 0.8–1.0 mg mL−1) in Ca2+-free ND96 (96 mM NaCl, 2 mM KCl, 3.8 mM MgCl2, 5 mM HEPES pH 7.4). Prior to

(5) Voltage clamp fluorometry in Xenopus laevis oocytes to study the voltage-sensing phosphataseBIO-PROTOCOLFebruary 20, 2025Victoria C. Young, Vamseedhar Rayaprolu, Susy C. Kohoutcatalog number: LS004177) 11. Collagenase type 3 (Worthington Biochemical Corporation, catalog number: LS004183) 12. Tetramethylrhodamine-6-maleimide (TMRM) (Abcam, catalog number: ab145471) 13. Sodium pyruvate (Fisher,

(6) Voltage clamp fluorometry in Xenopus laevis oocytes to study the voltage-sensing phosphataseBio ProtocFebruary 20, 2025Victoria C. Young, Vamseedhar Rayaprolu, Susy C. Kohoutcatalog number: LS004177) 11. Collagenase type 3 (Worthington Biochemical Corporation, catalog number: LS004183) 12. Tetramethylrhodamine-6-maleimide (TMRM) (Abcam, catalog number: ab145471) 13. Sodium pyruvate (Fisher,

(7) Downregulation of semaphorin 4A in keratinocytes reflects the features of non-lesional psoriasiseLifeDecember 31, 2024Miki Kume, Hanako Koguchi-Yoshioka, Shuichi Nakai, Yutaka Matsumura, Atsushi Tanemura, Kazunori Yokoi, Shoichi Matsuda, Yuumi Nakamura, Naoya Otani, Mifue Taminato, et al.,BD Biosciences,Cat# 555029, "Chemical compound, drug",Collagenase type III,Worthington Biochemical Corporation,Cat# LS004183, "Chemical compound, drug","CountBright Absolute Counting Beads, for flow cytometry",Thermo Fisher Scientific

(8) Downregulation of semaphorin 4A in keratinocytes reflects the features of non-lesional psoriasisElifeDecember 31, 2024Miki Kume, Hanako Koguchi-Yoshioka, Shuichi Nakai, Yutaka Matsumura, Atsushi Tanemura, Kazunori Yokoi, Shoichi Matsuda, Yuumi Nakamura, Naoya Otani, Mifue Taminato, et al.,BD Biosciences,Cat# 555029, "Chemical compound, drug",Collagenase type III,Worthington Biochemical Corporation,Cat# LS004183, "Chemical compound, drug","CountBright Absolute Counting Beads, for flow cytometry",Thermo Fisher Scientific

(9) Development of covalent chemogenetic K2P channel activatorsCell Chemical BiologyJuly 18, 2024Parker E. Deal, Haerim Lee, Abhisek Mondal, Marco Lolicato, Philipe Ribeiro Furtado de de Mendonça, Holly Black, Seil Jang, Xochina El-Hilali, Clifford Bryant, Ehud Y. Isacoff, et al.UCSF IACUC Protocol AN129690 and digested using collagenase (Worthington Biochemical Corporation, #LS004183, 0.8–1.0 mg/mL) in Ca2+-free ND96 (96 mM NaCl, 2 mM KCl, 3.8 mM MgCl2, 5 mM HEPES pH 7.4) immediately

(10) Hydrophobic residues in S1 modulate enzymatic function and voltage sensing in voltage-sensing phosphataseJournal of General PhysiologyJuly 1, 2024Vamseedhar Rayaprolu, Heini M. Miettinen, William D. Baker, Victoria C. Young, Matthew Fisher, Gwendolyn Mueller, William O. Rankin, John T. Kelley, William J. Ratzan, Lee Min Leong, et al.(catalog # LS004177; Worthington Biochemical Corporation) and 0.83 mg/ml collagenase type 3 (catalog # LS004183; Worthington Biochemical Corporation) made in Ca2+-free buffer. After digestion, the oocytes were washed

(11) Structure of the human K2P13.1(THIK-1) channel reveals a novel hydrophilic pore restriction and lipid cofactor sitebioRxivJune 29, 2024Shatabdi Roy-Chowdhury, Seil Jang, Fayal Abderemane-Ali, Fiona Naughton, Michael Grabe, Daniel L. MinorUCSF IACUC Protocol AN193390 and digested using collagenase (Worthington Biochemical Corporation, #LS004183, 0.8–1.0 mg/mL) in Ca2+-free ND96 (96 mM NaCl, 2 mM KCl, 3.8 mM MgCl2, 5 mM HEPES pH 7.4). Oocytes were

(12) Tumor microenvironment-induced FOXM1 regulates ovarian cancer stemnessCell death & diseaseMay 28, 2024Battistini C, Kenny HA, Zambuto M, Nieddu V, Melocchi V, Decio A, Lo Riso P, Villa CE, Gatto A, Ghioni M, et al.hyaluronidase (Merck, cat# H3884) and 1,000 U of collagenase type III (Worthington Biochemical, cat# LS004183) in DMEM (Euroclone, cat# ECM0103L), 10% FBS at 37 °C. The digested tissue was then centrifuged at 1400

(13) Reprogramming tumor-associated macrophages to outcompete endovascular endothelial progenitor cells and suppress tumor neoangiogenesisImmunityNovember 14, 2023Mytrang H. Do, Wei Shi, Liangliang Ji, Erik Ladewig, Xian Zhang, Raghvendra M. Srivastava, Kristelle J. Capistrano, Chaucie Edwards, Isha Malik, Briana G. Nixon, et al.Proteins","Chemicals, Peptides and Recombinant Proteins" Collagenase Type 3,Worthington Biochemical,LS004183 DNase I,Sigma-Aldrich, Percoll,Sigma-Aldrich,P1644-1L 2-Mercaptoethanol2-Mercaptoethanol,Thermo Scientific,21985023 Glutamax™ Supplement

(14) Development of covalent chemogenetic K2P channel activatorsbioRxivOctober 28, 2023Parker E. Deal, Haerim Lee, Abhisek Mondal, Marco Lolicato, Philipe Ribeiro Furtado de Mendonca, Holly Black, Xochina El-Hilali, Clifford Bryant, Ehud Y. Isacoff, Adam R. Renslo, et al.UCSF IACUC Protocol AN129690 and digested using collagenase (Worthington Biochemical Corporation, #LS004183, 0.8–1.0 mg/mL) in Ca2+-free ND96 (96 mM NaCl, 2 mM KCl, 3.8 mM MgCl2, 5 mM HEPES pH 7.4) immediately

(15) Tumor-associated macrophages expressing the transcription factor IRF8 promote T cell exhaustion in cancerImmunityNovember 1, 2022Briana G. Nixon, Fengshen Kuo, LiangLiang Ji, Ming Liu, Kristelle Capistrano, Mytrang Do, Ruth A. Franklin, Xiaodi Wu, Emily R. Kansler, Raghvendra M. Srivastava, et al.Proteins","Chemicals, Peptides, and Recombinant Proteins" Collagenase Type 3,Worthington Biochemical,LS004183 Dnase I,Sigma-Aldrich, Percoll,Sigma-Aldrich,P1644-1L Trizol LS,Invitrogen, OVA(257–264),Invivogen, Critical

(16) Oocytes maintain ROS-free mitochondrial metabolism by suppressing complex INatureJuly 1, 2022Rodríguez-Nuevo A, Torres-Sanchez A, Duran JM, De Guirior C, Martínez-Zamora MA, Böke E.digested in DMEM containing 25 mM HEPES and 2 mg ml−1 collagenase type III (Worthington Biochemical, LS004183) for 2 h at 37 °C with occasional swirling. Individual cells were separated from tissue fragments by

(17) Tracking cells in epithelial acini by light sheet microscopy reveals proximity effects in breast cancer initiationeLifeJuly 21, 2020Alladin A, Chaible L, Garcia Del Valle L, Sabine R, Loeschinger M, Wachsmuth M, Hériché JK, Tischer C, Jechlinger M.ThermoFisher Cat. # 15140122)) and 750 U of Collagenase Type 3 (Worthington Biochemical Corp Cat. # LS004183), 20 µg of Liberase (Roche Cat. # 5401020001) and incubated overnight at 37°C and 5%CO2. The glands

(18) In vivo Mouse Mammary Gland FormationBio-protocolJuly 5, 2020Sun H, Zhang X, Chan UI, Su SM, Guo S, Xu X, Deng C.catalog number: C8052) 22. Collagenase Type 3 (Worthington Biochemical Corporation, catalog number: LS004183) 23. Hyaluronidase (Sigma-Aldrich, catalog number: H3506) 24. Dispase II (Roche, catalog number: 04942078001) 25.

(19) Comparative analysis of exosome markers and extracellular vesicles between colorectal cancer and cancer-associated normal colonic mucosaPolish Archives of Internal MedicineJune 27, 2020Łukasz Zadka, Aleksandra Piotrowska, Agnieszka Opalińska, Katarzyna Haczkiewicz-Leśniak, Damian Grybowski, Ireneusz Ceremuga, Mariusz Chabowski, Piotr Dzięgiel(Worthington #CLS‑3, Worthington Biochemical Corporation, Lakewood, California, United States, Cat. No. LS004183, 5 gm). Incuba‑ tion was carried out in a shaking water bath set at 37 °C with gentle shaking for a

(20) Polynuclear Ruthenium Amines Inhibit K2P Channels via a “Finger in the Dam” MechanismCell Chemical BiologyMay 1, 2020Lianne Pope, Marco Lolicato, Daniel L. MinorUCSF IACUC Protocol AN129690 and digested using collagenase (Worthington Biochemical Corporation, #LS004183, 0.7–0.8 mg mL−1) in Ca2+-free ND96 (96 mM NaCl, 2 mM KCl, 3.8 mM MgCl2, 5 mM HEPES pH 7.4) immediately

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Collagenase, Type 3 from Worthington Biochemical Corporation

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Worthington Biochemical Corporation

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Collagenase, Type 3 from Worthington Biochemical Corporation

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