Elastase, Suspension LS002280 from Worthington Biochemical Corporation

Product Specs
ItemElastase, Suspension
CompanyWorthington Biochemical Corporation
Price $1,065.00Supplier Page View Company Product Page
Catalog NumberLS002280
Source/Expression SystemPorcine Pancreas
StorageStore at 2-8°C. DO NOT FREEZE
Activity≥3 units per mg protein
Size1 gm

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Worthington Biochemical Corporation

Lakewood, New Jersey 8701

United States

Phone: 1.800.445.9603
1.732.942.1660

Email: custservice@worthington-biochem.com

Company Profile

Website: worthington-biochem.com

(1) Immortalization of Human Adult Alveolar Epithelial Cells to Study Environmental Exposures of the Distal LungCurr ProtocApril 30, 2026Evelyn Tran, Laurence St. Pierre, Diego Velarde, Bianca Dal Bó, Juan Ramon Alvarez, Kweku Ofosu-Asante, Nazarius S. Lamango, Yong Huang, Crystal N. Marconett, Zea Borok, et al.(DMEM‐F12) base medium (Corning, 10‐092‐CV)Diluted elastase solution, 13 U/ml (Worthington Biochemical, LS002280; see “Reagent preparation” section below)Hank's balanced salt solution without calcium and magnesium

(2) Triggering AHR resolves TGF-β1 induced fibroblast activation and promotes AT1 cell regeneration in alveolar organoidsCommunications biologyJuly 9, 2025Hagan AS, Williams S, Mathison CJN, Yan S, Nguyen B, Federe GC, Kuzu G, Siefert JC, Hampton J, Chichkov V, et al.contained: 450 U/mL Collagenase Type I (Gibco; 17100–017), 4 U/mL Elastase (Worthington Biochemical Company; LS002279), Dispase 5 U/mL (BD Biosciences; 354235), and 0.33 U/mL DNaseI (Roche; 10104159001) dissolved in DMEM/F12

(3) Single cell profiling of human airway identifies tuft-ionocyte progenitor cells displaying cytokine-dependent differentiation bias in vitroNat CommunJune 4, 2025Viral S. Shah, Avinash Waghray, Brian Lin, Atharva Bhagwat, Isha Monga, Michal Slyper, Bruno Giotti, Sunghyun Kim, Dawei Sun, Ke Xu, et al.dispase (2.5 units/ml Worthington Biochemical LS02100), elastase (2 units/ml Worthington Biochemical LS002279), pronase (1 mg/ml Roche11 459 643 001), DNAse (1 unit/ml, Worthington LK003170) and Y-27632 inhibitor

(4) MMP9(High) Neutrophils are Critical Mediators of Neutrophil Extracellular Traps Formation and Myocardial Ischemia/Reperfusion InjuryAdvanced science (Weinheim, Baden-Wurttemberg, Germany)June 1, 2025Hu S, Zhang F, Wang J, Zhang J, Li C, Lyu Y, Wang Y, Huang R, Gao Y, Yang H, et al.II collagenase (1.5 mg mL−1; LS004202, Worthington Biochemical Corporation), elastase (0.25 mg mL−1; LS002274, Worthington Biochemical Corporation), and DNase I (0.5 mg mL−1; LS002145, Worthington Biochemical Corporation)

(5) Integrated multiomic analysis identifies TRIP13 as a mediator of alveolar epithelial type II cell dysfunction in idiopathic pulmonary fibrosisBiochimica et Biophysica Acta (BBA) - Molecular Basis of DiseaseMarch 1, 2025Laurence St. Pierre, Asres Berhan, Eun K. Sung, Juan R. Alvarez, Hongjun Wang, Yanbin Ji, Yixin Liu, Haoze Yu, Angela Meier, Kamyar Afshar, et al.Salt Solution (HBSS) without calcium or magnesium (#21-021-CV, VWR) containing 13 U/mL of elastase (#LS002280, Worthington Biochemical Corp) and incubated for 30 min at 37 °C. Tissue homogenate was serially filtered

(6) Long-term maintenance of patient-specific characteristics in tumoroids from six cancer indicationsScientific ReportsJanuary 31, 2025Colin D. Paul, Chris Yankaskas, Pradip Shahi Thakuri, Brittany Balhouse, Shyanne Salen, Amber Bullock, Sylvia Beam, Anthony Chatman, Sybelle Djikeng, Xiaoyu Jenny Yang, et al.dispase II (Thermo Fisher Scientific, cat# 17105-041), 0–20 U/ml elastase (Worthington Biochemical, cat# LS002279), 0-200 U/ml hyaluronidase (STEMCELL Technologies, Vancouver, BC, CAN, cat# 07462), and 0-500 U/ml DNase I

(7) Identifying sex similarities and differences in structure and function of the sinoatrial node in the mouse heartFrontiers in MedicineDecember 5, 2024Zeyuan Yin, Eleonora Torre, Manon Marrot, Colin H. Peters, Amy Feather, William G. Nichols, Sunil Jit R. J. Logantha, Areej Arshad, Simran Agnes Martis, Nilay Tugba Ozturk, et al.collagenase Type II (1,064 U; L5004177; Worthington Biochemical, New Jersey, United States), elastase (9 U; LS002279; Worthington Biochemical, New Jersey, United States), and protease (65.2 μL of 1 mg/100 μL ddH2O; P463;

(8) PAI-1 Regulates the Cytoskeleton and Intrinsic Stiffness of Vascular Smooth Muscle CellsArteriosclerosis, thrombosis, and vascular biologyOctober 1, 2024Khoukaz HB, Vadali M, Schoenherr A, Ramirez-Perez FI, Morales-Quinones M, Sun Z, Fujie S, Foote CA, Lyu Z, Zeng S, et al.elastase (0.75 units/mL) from Worthington Biochemical, Lakewood, NJ, USA (#LS004174, #LS003570, and #LS002279, respectively). After removing the adventitia, SMCs were isolated and cultured as described.20 After

(9) Smooth muscle-derived adventitial progenitor cells promote key cell type transitions controlling plaque stability in atherosclerosis in a Klf4-dependent mannerbioRxiv : the preprint server for biologyJuly 19, 2023Dubner AM, Lu S, Jolly AJ, Strand KA, Mutryn MF, Hinthorn T, Noble T, Nemenoff RA, Moulton KS, Majesky MW, et al."Trypsin Inhibitor, Soybean",Worthington Biochemical Corporation,LS003571 Elastase Suspension,Worthington Biochemical Corporation,LS002280 Collagenase Type 2,Worthington Biochemical Corporation,LS004177 Sudan IV,Sigma-Aldritch,198102-25G Critical

(10) C781, a β-Arrestin Biased Antagonist at Protease-Activated Receptor-2 (PAR2), Displays in vivo Efficacy Against Protease-Induced Pain in MiceThe Journal of PainApril 1, 2023Moeno Kume, Ayesha Ahmad, Stephanie Shiers, Michael D. Burton, Kathryn A. DeFea, Josef Vagner, Gregory Dussor, Scott Boitano, Theodore J. Pricepreviously described for 2AT and C781.6,7,20,21,46,54 For in vitro studies, neutrophil elastase (NE; cat# LS002274) and trypsin (cat# LS003703) were purchased from Worthington Biochemical Corporation, Lakewood New Jersey.

(11) Population-wide gene disruption in the murine lung epithelium via AAV-mediated delivery of CRISPR-Cas9 componentsMolecular therapy. Methods & clinical developmentDecember 8, 2022Chen H, Durinck S, Patel H, Foreman O, Mesh K, Eastham J, Caothien R, Newman RJ, Roose-Girma M, Darmanis S, et al.cocktail containing collagenase/dispase (cat. no. 11097113001, Roche, Switzerland), elastase (cat. no. LS002279, Worthington Biochemical, NJ), and DNaseI (cat. no. 10104159001, Roche) in DMEM/F12 medium. Lungs were

(12) Mesothelial cell-derived antigen-presenting cancer-associated fibroblasts induce expansion of regulatory T cells in pancreatic cancerCancer cellJune 13, 2022Huang H, Wang Z, Zhang Y, Pradhan RN, Ganguly D, Chandra R, Murimwa G, Wright S, Gu X, Maddipati R, et al.LS004176 Collagenase III,Worthington Biochemical,Cat# LS004182 Elastase,Worthington Biochemical,Cat# LS002279 Critical Commercial Assays,Critical Commercial Assays,Critical Commercial Assays Fixable Viability

(13) Inducible Prmt1 ablation in adult vascular smooth muscle leads to contractile dysfunction and aortic dissectionExperimental & Molecular MedicineOctober 11, 2021Jung-Hoon Pyun, Byeong-Yun Ahn, Tuan Anh Vuong, Su Woo Kim, Yunju Jo, Jaehyung Jeon, Seung Ho Baek, Jaewon Kim, Sungsu Park, Gyu-Un Bae, et al.The medial layer of the aorta was separated from the adventitia by elastase (Worthington Biochemical, LS002279) and collagenase (Worthington Biochemical, LS04177) treatment. To preserve smooth muscle cell characteristics,

(14) Class II phosphatidylinositol 3-kinase α and β isoforms are required for vascular smooth muscle Rho activation, contraction and blood pressure regulation in miceThe Journal of Physiological SciencesDecember 1, 2020Shahidul Islam, Kazuaki Yoshioka, Sho Aki, Kazuhiro Ishimaru, Hiroki Yamada, Noriko Takuwa, Yoh Takuwa(Type II) (#LS004202, Worthington Biochemical, Lakewood, NJ, USA), 0.75 unit/mL elastase (Type III) (#LS002279, Worthington Biochemical) and 0.1% trypsin inhibitor (#LS003570, Worthington Biochemical) for 30 min,

(15) Periprocedural Hydrogen Sulfide Therapy Improves Vascular Remodeling and Attenuates Vein Graft DiseaseJournal of the American Heart AssociationNovember 17, 2020Kip P, Tao M, Trocha KM, MacArthur MR, Peters HAB, Mitchell SJ, Mann CG, Sluiter TJ, Jung J, Patterson S, et al.of 1 mg/mL Collagenase type 2 (Gibco, cat# 17101015), 0.24 mg/mL Elastase (Worthington Biochemical, LS002279), 1 mg/mL Soybean Trypsin Inhibitor (Worthington Biochemical, LS0033570), 1% penicillin/streptomycin

(16) Mesenchymal Stem Cells Exhibit Both a Proinflammatory and Anti-Inflammatory Effect on Saccular Aneurysm Formation in a Rabbit ModelStem Cells InternationalJuly 22, 2019Michael B. Avery, Brooke L. Belanger, Amy Bromley, Arindom Sen, Alim P. Mithaangiocatheter, and approximately 35 units of elastase (Worthington Biochemical Corporation, Cat. No. LS002279) was infused into the lumen. The angiocatheter entry site was secured with a silk tie. After twenty

(17) ABL kinase inhibition sensitizes primary lung adenocarcinomas to chemotherapy by promoting tumor cell differentiationOncotargetMarch 8, 2019Khatri A, Gu JJ, McKernan CM, Xu X, Pendergast AM.collagenase (ThermoFisher: 17100017), 100 μL elastase (4U/mL) (Worthington Biochemical Corporation: LS002279), 300 μL dispase (5U/mL) (BD Biosciences: 354235), and 200 μL Dnase I (0.33U/mL) (Roche: 10104159001).

(18) FoxA1 and FoxA2 drive gastric differentiation and suppress squamous identity in NKX2-1-negative lung cancereLifeNovember 26, 2018Camolotto SA, Pattabiraman S, Mosbruger TL, Jones A, Belova VK, Orstad G, Streiff M, Salmond L, Stubben C, Kaestner KH, et al.I,ThermoFisher Scientific,17100017, "Commercial assay, kit",Elastase,Worthington Biochemical Corporation,LS002280, "Commercial assay, kit",Dispase,Corning,354235, "Commercial assay, kit",Deoxyribonuclease I,Sigma-Aldrich,DN25, "Commercial

(19) PAR2 (Protease-Activated Receptor 2) Deficiency Attenuates Atherosclerosis in MiceArteriosclerosis, thrombosis, and vascular biologyJune 1, 2018Jones SM, Mann A, Conrad K, Saum K, Hall DE, McKinney LM, Robbins N, Thompson J, Peairs AD, Camerer E, et al.mg/mL soybean trypsin inhibitor (Worthington Biochemical LS003570), elastase (Worthington Biochemical LS002279, final concentration ~1 U/mL), and 1% penicillin/streptomycin (Gibco) made in sterile 1x phosphate buffered

(20) Niche-mediated BMP/SMAD signaling regulates lung alveolar stem cell proliferation and differentiationDevelopment (Cambridge, England)May 11, 2018Chung MI, Bujnis M, Barkauskas CE, Kobayashi Y, Hogan BLM.collagenase type I (450 U/ml; Gibco #17100-017), elastase (4 U/ml; Worthington Biochemical Corporation #LS002279), dispase (5 U/ml; BD Biosciences #354235) and DNaseI (0.33 U/ml) in DMEM/F12. Lung lobes were separated,

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Elastase, Suspension from Worthington Biochemical Corporation

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Worthington Biochemical Corporation

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Elastase, Suspension from Worthington Biochemical Corporation

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