Collagenase, Type 1 LS004197 from Worthington Biochemical Corporation

Product Specs
ItemCollagenase, Type 1
CompanyWorthington Biochemical Corporation
Price $900.00Supplier Page View Company Product Page
Catalog NumberLS004197
Source/Expression SystemClostridium histolyticum
StorageStore at 2-8°C
Activity≥125 units per mg dry weight
Size5 gm

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Worthington Biochemical Corporation

Lakewood, New Jersey 8701

United States

Phone: 1.800.445.9603
1.732.942.1660

Email: custservice@worthington-biochem.com

Company Profile

Website: worthington-biochem.com

(1) Intestinal neutral ceramidase exacerbates MASH pathogenesiseGastroenterologyJune 30, 2026Ting Wang, Liang Chen, Chao Lei, Xiaotong Song, Amanguli Tuohongerbieke, Jing Feng, Regina Gasparetto, Xiang Zhang, Craig J McClain, Yi Tan, et al.described previously.19 27 Lung was cut and digested in 3 mL medium (RPMI 1640 containing collagenase I) (LS004197, Worthington Biochemical, Lakewood, New Jersey, USA) and DNase I (50 ug/mL; LS006328, Worthington Biochemical)

(2) Mitochondrial carrier SLC25A34 links clock, diet, and temperature control of interorganellar lipid cyclingbioRxivJune 3, 2026Iuliia Karavaeva, Astrid Linde Basse, Samuel A. J. Trammell, Mohammed Faiz Hussain, Lasse Kruse Markussen, Jesper F. Havelund, Marie Sophie Isidor, Hannah J. Richter, Sabina Chubanava, Yann Deleye, et al.supplemented with 2% BSA (Sigma-Aldrich, A7030) and 0.2% collagenase type I (Worthington Biochemical Corp., LS004197) at 37°C for 40 min. The digests were centrifuged (5 min, 400 g) to obtain the stromal vascular fraction

(3) Isolation of Extracellular Vesicles From Mouse Brown Adipose Tissue Secretome Using Size-Exclusion ChromatographyFASEB JMay 15, 2026Parth Pardeshi, Daniel Velasquez-Ramirez, Juan David Salazar-Gil, Tatiana Cuellar-Gaviria, Lisa A. Baer, Natalia Higuita-Castro, Kristin I. Stanford(Roche, 11376497001)Dexamethasone (Sigma, D‐4902)Collagenase type 1 (Worthington Biochemical Corporation, LS004197)Sterile Phosphate buffered saline (PBS)Sterile ultrapure distilled water (UPDW)Sterile filtered 0.5

(4) A convergent uPAR-positive tumor ecosystem creates broad vulnerability to CAR T cell therapyCellMay 14, 2026Zeda Zhang, Yu-Jui Ho, Xin Fang, Minseo Kim, Marguerite Li, Wei Luan, Clemens Hinterleitner, Sascha Haubner, Friederike Kogel, Edwin C. Pratt, et al."LIVE/DEAD™ Fixable Aqua Dead Cell Stain Kit, for 405 nm excitation",Thermo Fisher Scientific,Cat# L34957 Collagenase Type 1,Worthington Biochemical Corporation,Cat# LS004197 TaqMan Reverse Transcription Reagents,Applied Biosystems,Cat# N8080234 SYBR Green Universal Master

(5) Isolation of Extracellular Vesicles From Mouse Brown Adipose Tissue Secretome Using Size‐Exclusion ChromatographyFASEB JApril 24, 2026Parth Pardeshi, Daniel Velasquez‐Ramirez, Juan David Salazar‐Gil, Tatiana Cuellar‐Gaviria, Lisa A. Baer, Natalia Higuita‐Castro, Kristin I. Stanford(Roche, 11376497001)Dexamethasone (Sigma, D‐4902)Collagenase type 1 (Worthington Biochemical Corporation, LS004197)Sterile Phosphate buffered saline (PBS)Sterile ultrapure distilled water (UPDW)Sterile filtered 0.5

(6) Boosting Sensory Nerve‐to‐Bone Interactions Enhances Hedgehog Mediated Calvarial Bone RepairAdvanced ScienceApril 20, 2026Zhao Li, Xin Xing, Beicheng Du, Myles Zhou, Austin Z. Chen, Mary Archer, Chunbao Rao, Manyu Zhu, Masnsen Cherief, Aaron W. Jamesmixture containing collagenase type I (1 mg/mL; Worthington Biochemical Corporation, Lakewood, NJ, USA, LS004197) and collagenase type II (1 mg/mL; Worthington Biochemical Corporation, LS004177). Cell fractions (from

(7) Large adipocytes increase vesicle-mediated lipid release and promote breast cancer malignancyCell ReportsMarch 24, 2026Garrett F. Beeghly, Irina Kopyeva, Jenny Deng, Marlee I. Pincus, Rohan R. Varshney, Dilip D. Giri, Domenick J. Falcone, Michael C. Rudolph, Marc A. Antonyak, Neil M. Iyengar, et al.discarded. The reserved tissue was then digested in 1.5 mg/mL collagenase type I (Worthington Biochemical #LS004197) in Hank’s Buffered Salt Solution (Gibco #14065056) supplemented with 1% (w/v) bovine serum albumin

(8) Pathologic Th1-Treg Cells Exacerbate Acute Lung Injury and Lethality in SepsisCellsMarch 14, 2026Takuya Murao, Atsushi Murao, Monowar Aziz, Ping WangCells Lung tissues were minced into small pieces and incubated with 1 mg/mL collagenase type I (Cat. No. LS004197, Worthington Biochemical, Lakewood, NJ, USA) at 37 °C for 30 min with periodic agitation. The digested

(9) The Recycling Collagen Receptor uPARAP Is a Unique Mediator of Stromal Drug Delivery to Carcinoma CellsMolecular cancer therapeuticsJanuary 2, 2026Nørregaard KS, Larsen IME, Jürgensen HJ, Blomquist MH, Çakilkaya P, Metrangolo V, Perlado AM, Krigslund O, Gårdsvoll H, Poulsen TT, et al.cell culture and cell-based assays in vitro, reagents included collagenase type I (CAS no. 9001-12-1, cat. no. LS004197), DNAse I (CAS no. 9003-98-9, cat. no. LS002006, Worthington Biochemical), neutral protease (dispase,

(10) Sympathetic nerve inhibition enhances calvarial bone repair via senescent macrophage-induced osteogenesis and angiogenesisCell Death DiscovDecember 10, 2025Lei Zhao, Zhaoning Xu, Peiru Zhao, Zhiying Pang, You You, Chao Wu, Di Zhu, Meiling Su, Ning Zhang, Jian Luo, et al.with a mixture of collagenase Type I/II (1 mg/mL, Worthington Biochemical Corporation, Lakewood, NJ; LS004197 and LS004177) and Dispase II (2 mg/mL) for three rounds of 15 min each. Three animals were included

(11) Age-dependent immune responses and resident cell dynamics in young mice following pneumoniaiScienceNovember 26, 2025Sylvia N. Michki, Javier V. Perez, Dharma A. Varapula, Aaron J. Thomas, Tiffaney L. Vincent, Benjamin D. Singer, Jarod A. Zepp, Sharon A. McGrath-Morrowpooled lungs). Lungs were dissociated using 1.2 mg/mL Collagenase Type 1 (Worthington Biochemical, LS004197), 5U/mL Dispase (Corning, 354235), 0.01 mg/mL DNAse I (Stem Cell Technologies, 07900), 1× PBS). ACK

(12) Identification Of Protein Cargo in Extracellular Vesicles from Macrophages in Progressing and Regressing TumorsbioRxivNovember 9, 2025Yining Zhu, Jiaxi Cai, Raymond T. Suhandynata, Jack D. Buiblades and then enzymatically via type 1A collagenase (1 mg/mL) (Worthington Biochemical Corporation LS004197) digestion for 30 minutes at 37 degrees Celsius. The tumor single-cell suspension was passed through

(13) Adipose-mimetic granular hydrogels uncover biophysical cues driving breast cancer invasionbioRxivOctober 25, 2025Brianna K. Knode, Garrett Farrell Beeghly, Brittany E. Schutrum, Dong Wang, Yitong Zheng, Alice Battistella, Ruchi Goswami, Chi-Yong Eom, Aline Bozec, Jochen Guck, et al.Floating tissue pieces were then digested in 1.5 mg/mL collagenase type I (Worthington Biochemical #LS004197) in Hank’s Buffered Salt Solution (Gibco #14065056) supplemented with 1% (w/v) bovine serum albumin

(14) Primate resident memory T cells activate humoral and stromal immunityImmunityOctober 14, 2025Vineet Joag, Benjamin N. Bimber, Clare F. Quarnstrom, Venkata S. Bollimpelli, Jason M. Schenkel, Kathryn A. Fraser, Mario Bertogliat, Andrew G. Soerens, J. Michael Stolley, Stephen D. O’Flanagan, et al.University",N/A RNAlater,Qiagen,AM7020 Trizol,Sigma-Aldrich,T9424 Collagenase Type I,Worthington Biochemical Corporation,LS004197 Collagenase Type IV from Clostridium histolyticum,Sigma-Aldrich,C5138 DNase I,Sigma-Aldrich,D5025 "Dithioerythritol, 99

(15) MK2 deficiency decreases mortality in male mice during the inflammatory phase after myocardial infarctionPhysiological ReportsSeptember 19, 2025Joëlle Trépanier, Sherin A. Nawaito, Pramod Sahadevan, Fatiha Sahmi, Natacha Duquette, Danielle Gélinas, Marc‐Antoine Gillis, Yanfen Shi, Cynthia Torok, Marie‐Élaine Clavet‐Lanthier, et al.MgSO4, 1 mM CaCl2, 1 mg/mL BSA, 133 U/mL collagenase type IA (Worthington Biochemical Corporation #LS004197), 1 mg/mL trypsin (Gibco #27250‐018), 20 μg/mL pancreatin (Sigma‐Aldrich #P3292), pH 7.4) with gentle

(16) Protocol for establishing genetically engineered murine lung organoids mimicking cell plasticity and regenerationSTAR ProtocolsSeptember 1, 2025Bongjun Kim, Jae-Il Park25200056 GlutaMAX,Gibco,Cat# 35050061 HEPES (1 M),Gibco,Cat# 15630080 Collagenase type I,Worthington Biochemical,Cat# CLS-1 LS004197 Elastase,Worthington Biochemical,Cat# ESL LS002294 DNase I,Worthington Biochemical,Cat# D LS002007 Bovine

(17) MSC1 Cells Suppress Colorectal Cancer Cell Growth via Metabolic Reprogramming, Laminin-Integrin Adhesion Signaling, Oxidative Stress Resistance, and a Tumor-Suppressive SecretomeBiomedicinesJune 19, 2025Panagiota-Angeliki Galliou, Niti Argyri, Papaioannou Maria, George Koliakos, Nikolaos A. Papanikolaoufragments, and enzymatically digested in 0.3% collagenase type I (Worthington Biochemical Corp., Cat# LS004197, Lakewood, NJ, USA), 0.03% hyaluronidase (Worthington Biochemical Corp., Cat# LS002592), 1% penicillin/streptomycin

(18) Proximal tubule pannexin 1 contributes to mitochondrial dysfunction and cell death during acute kidney injuryAmerican Journal of Physiology-Renal PhysiologyJune 1, 2025Nabin Poudel, Shuqiu Zheng, Nataliya Skrypnyk, Sun-Sang J. Sung, Eibhlin Goggins, William T. Nash, Caitlin Pavelec, Marissa Yee, Ishaq Balogun, Cristopher B. Medina, et al.were harvested, and cortical pieces were digested with collagenase Type I (Worthington Biochemical # LS004197) in the presence of soybean trypsin inhibitor (Gibco # 17075029) at 37° C for 25 minutes. The digestion

(19) Host responses to S. pneumoniae in wild type and Mertk mutant micePLoS OneApril 16, 2025Matthew K. McPeek, Jessica R. Martin, John C. Gomez, Yitong Li, Hong Dang, H. Shelton Earp, Claire M. Doerschukwith 0.5 mL of RPMI media containing 5mg/mL collagenase I (Worthington Biochemical Corporation, Cat. LS004197) and 1mg/mL deoxyribonuclease I (Worthington, Cat. LS002140) followed by 0.3mL 1% (wt/vol) low melting

(20) Collaboration between interleukin-7 and -15 enables adaptation of tissue-resident and circulating memory CD8+ T cells to cytokine deficiencyImmunityMarch 11, 2025Nicholas N. Jarjour, Talia S. Dalzell, Nicholas J. Maurice, Kelsey M. Wanhainen, Changwei Peng, Stephen D. O’Flanagan, Taylor A. DePauw, Katharine E. Block, William J. Valente, K. Maude Ashby, et al.recombinant proteins","Chemicals, peptides, and recombinant proteins" Collagenase I,Worthington Biochemical,LS004197 Collagenase IV,Thermo Fisher Scientific,17104019 Dithioerythritol,EMD Millipore,233152 Percoll,GE Healthcare

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Collagenase, Type 1 from Worthington Biochemical Corporation

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Worthington Biochemical Corporation

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Collagenase, Type 1 from Worthington Biochemical Corporation

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