Fig 1: GTPBP4 promotes prepubertal goat oocytes maturation in vitro. (A) Localisation of GTPBP4 during goat oocyte maturation. Green, GTPBP4; blue, DNA; white arrow indicates cumulus cell. Bar = 50 μm. (B) Prepubertal goat oocytes after 24 h of treatment with different concentrations of hRec‐GTPBP4. Black arrow indicates first polar body. Bar = 100 μm. (C) Nuclear conformation were used to define the cell cycle of prepubertal goat oocytes. Bar = 50 μm. (D) After 24 h maturation, the percentage of different meiotic stages of prepubertal goat oocytes in the 0, 100, 300 and 500 ng/mL hRec‐GTPBP4 group. The data were displayed as mean ± SD of three independent experiments. 0 ng/mL, n = 53 oocytes; 100 ng/mL, n = 55 oocytes; 300 ng/mL n = 51 oocytes; and 500 ng/mL n = 55 oocytes. *, p < 0.05, **, p < 0.01. (E) Immunofluorescence staining results of RPL7A in prepubertal goat MII oocytes. Green, RPL7A; blue, DNA. Bar = 50 μm. (F) The fluorescence intensity of RPL7A treated with of 0 ng/mL (n = 32 oocytes) and 300 ng/mL (n = 38 oocytes) hRec‐GTPBP4. The data were displayed as mean ± SD of three independent experiments. ***, p < 0.001. (G) Immunofluorescence staining results of protein synthesis in prepubertal goat MII oocytes. Green, protein synthesis. Bar = 50 μm. (H) The fluorescence intensity of protein synthesis treated with of 0 ng/mL (n = 23 oocytes) and 300 ng/mL (n = 21 oocytes) hRec‐GTPBP4. The data were displayed as mean ± SD of three independent experiments. **, p < 0.01. (I) Mitochondrial distribution pattern in prepubertal goat MII oocytes. Red, mitochondria; blue, DNA. Bar = 50 μm. (J) The uniform distribution ratios of mitochondria treated with 0 ng/mL (n = 32 oocytes) and 300 ng/mL (n = 38 oocytes) hRec‐GTPBP4. The data were displayed as mean ± SD of three independent experiments. **, p < 0.01. (K) Representative images of IVF blastocysts derived from oocytes cultured IVM medium containing 0 and 300 ng/mL hRec‐GTPBP4. The red asterisk indicates blastocyst. Bar = 200 μm, 100 μm. (L) The blastocyst percentages derived from 0 ng/mL (n = 64 oocytes) and 300 ng/mL (n = 70 oocytes) hRec‐GTPBP4. The data were displayed as mean ± SD of three independent experiments. *, p < 0.05. The blastocyst rate were computed on the number of cleavage embryos.
Fig 2: Translational activity of prepubertal goat GV and MII oocytes. (A) Graph showing the number of translationally active genes (red) and translationally repressed genes (blue) in GV oocytes from adult and prepubertal goat. (B) Veen plot shows the overlap of translationally active genes (red) and translationally repressed genes (blue) between adult and prepubertal goat GV oocytes. (C) Representative GO BP terms enrichment of prepubertal‐specific translationally active genes (red) and translationally repressed genes (blue) in GV oocytes. (D) Graph showing the number of translationally active genes (red) and translationally repressed genes (blue) in MII oocytes from adult and prepubertal goat. (E) Veen plot shows the overlap of translationally active genes (red) and translationally repressed genes (blue) between adult and prepubertal goat MII oocytes. (F) Representative GO BP terms enrichment of prepubertal‐specific translationally active genes (red) and translationally repressed genes (blue) in MII oocytes. (G) Veen plot shows the overlap of translational dynamics abnormal genes in prepubertal goat oocytes and prepubertal‐specific translationally repressed genes. (H) The overlapped genes of regulating organelle function, spindle organisation and chromosome segregation showed in Figure 5G. (I) The expression results of GTPBP protein family at transcriptional and translational level during goat oocyte maturation. (J) The biological function of GTPBP4 in somatic cells and its potential role in oocytes.
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