Recombinant Proteinase K Solution (20 mg/mL) AM2548 from Thermo Fisher Scientific

Product Specs
ItemRecombinant Proteinase K Solution (20 mg/mL)
CompanyThermo Fisher Scientific
Price Inquire
Catalog NumberAM2548
FormatLiquid
Size5 x 1.25 mL
TypeProteinase K
Concentration20 mg/mL
HostT. album

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Thermo Fisher Scientific

168 Third Avenue

Waltham, MA 02451

United States

Phone: 800-678-5599

Company Profile

Website: www.thermofisher.com

(1) Protocol for clonal isolation of gene-edited hiPSCs using droplet and microfluidic sortingSTAR ProtocApril 24, 2026Hemanta Sarmah, Grazia Iannello, Ryan Wantroba, Christopher Wu, Juan Idiarte, Adonary Munoz, Olivier Déry, Elena Rubio de la Torre, Ekaterina Lebayle, Serena Seminara, et al.,Integrated DNA Technologies,Cat# 1081061 Synthetic guide RNAs (gRNAs),Synthego, Proteinase K,Invitrogen,Cat# AM2548 Matrigel GFR Membrane Matrix,Corning,Cat# 356230/354230 ,, Experimental models: Cell lines,Experimental models

(2) Systematic discovery of pro- and anti-HIV host factors in primary human CD4+ T cellsCellApril 20, 2026Ujjwal Rathore, Eli Dugan, Hunter Thornton, Vigneshwari Easwar Kumar, Rama Dajani, Ryan C. Burdick, Janet M. Young, Zachary Steinhart, Reanna Lao, Krista A. Delviks-Frankenberry, et al.,Thermo Fisher Scientific,Cat# AM9516 Invitrogen Recombinant Proteinase K Solution (20mg/mL),Thermo Fisher Scientific,Cat# AM2548 KAPA HiFi HS RM,Roche,Cat# 07958935001 Lipofectamine 3000 Transfection Reagent,Thermo Fisher Scientific,Cat#

(3) Integrating Microscopy Methods to Study Gene and Protein Expression alongside Metal Ion Distribution and Speciation: A Case Study of Iron within Pyramidal Neurons from Distinct Hippocampal CA1 SubregionsChemical & Biomedical ImagingMarch 23, 2026Gaewyn Ellison, Rhiannon E. Boseley, Meg Willans, Sarah Williams, Evelyn S. Innes, Paige Barnard, Julia Koehn, Somayra S. A. Mamsa, Paul Quinn, Daryl L. Howard, et al.15 min) using Tris-EDTA buffer (eBiosciences 00–4956–58) and proteinase K digestion (Thermo Fisher AM2548, 0.1 ug/mL final concentration, 37 °C for 15 min). Target detection was performed with the whole transcriptome

(4) Protocol for lentiviral engineering and multi-omic characterization of human kidney tubuloidsSTAR ProtocolsMarch 20, 2026Giulia Perticari, Maroussia M.P. Ganpat, Jarno Drost11873580001 EGTA,Sigma-Aldrich,Cat# 324626 NP-40,Sigma-Aldrich,Cat# 9016-45-9 Proteinase K,Invitrogen,Cat# AM2548 Sodium chloride,VWR,Cat# 27810.295 ProteinA-MN,Zeller et al.5,N/A Calcium chloride,Sigma Aldrich,Cat#

(5) MFF budding from mitochondria regulates melanosome size and maturationNature CommunicationsMarch 14, 2026Ana Paula Magalhães Rebelo, Aurora Maracani, Samuele Greco, Federica Dal Bello, Lucia Santorelli, Marco Gerdol, Alberto Pallavicini, Tomas Knedlik, Sara Schiavon, Luca Scorrano, et al.(Bio-Rad, Cat. No. 500000). MMERF were treated on ice for 15 min with proteinase K (Invitrogen, Cat. No. AM2548) in a final concentration of 15 µg/ml diluted in mitochondrial isolation buffer. Proteinase K was subsequently

(6) Type 2 lymphocytes restrict type 3 lymphocytes during liver fibrosis and colocalize in fibroblast nichesSci AdvMarch 13, 2026Julia Sbierski-Kind, Kelly M. Cautivo, Julia Nilsson, Johanna C. Wagner, Madelene W. Dahlgren, Nathan Ewing Crystal, Maria McClave, Nicholas M. Mroz, Marlene Ganslmeier, Carlos O. Lizama, et al.(pH 9) for 20 min, followed by 15-min incubation at 37°C in proteinase K (1 μg/ml; Invitrogen, no. AM2548). The Whole Transcriptome Atlas probe was applied to each slide and hybridized overnight at 37°C in

(7) DCPS modulates TDP-43-linked neurodegeneration through P-body-mediated RNA decayNeuronMarch 9, 2026Yingzhi Ye, Zhe Zhang, Yu Xiao, Chengzhang Zhu, Noelle Wright, Julie Asbury, Yongxin Huang, Weiren Wang, Laura Gomez-Isaza, Juan C. Troncoso, et al.,ThermoFisher Scientific,Cat #R0161 RNaseOUT,ThermoFisher Scientific,Cat #10777019 Proteinase K,ThermoFisher Scientific,Cat #AM2548 RNase H,New England Biolabs,Cat #M0297S RNase A/T1,ThermoFisher Scientific,Cat #EN0551 UltraPure

(8) Protocol for dual spatial transcriptomic profiling of infected tissuesSTAR ProtocolsMarch 1, 2026Hao Zhou, Ankush Tyagi, Maxine McClain, Esperanza Anguiano, Serena Abbondante, Eric Pearlman, Mihaela Gadjeva244520 10% formalin,Thermo Fisher Scientific,Cat# SF100-4 Proteinase K,Thermo Fisher Scientific,Cat# AM2548 100% deionized formamide,Thermo Fisher Scientific,Cat# AM9342 20×SSC,Invitrogen,Cat# AM9763 10% Tween

(9) Stress controls heterochromatin inheritance via histone H3 ubiquitylationNatureFebruary 19, 2026Bharat Bhatt, Yi Wei, Ashis Kumar Pradhan, Jothy Dhakshnamoorthy, Martin Zofall, Hua Xiao, Drisya Vijayakumari, Shweta Jain, Hernan Diego Folco, Hongyun Qi, et al.buffer was added to make a final volume of 150 μl. Then 20 μg of proteinase K (Thermo Fisher Scientific, AM2548) was added to both immunoprecipitation and input samples, and the mixtures were incubated at 37 °C for

(10) Topologically Associating Domains Define the 3D Genome Architecture of Mouse Totipotent-like Stem CellsJournal of Biological ChemistryFebruary 5, 2026Yu Fu, Wenyu Ma, Jin Han, Wenhui Zhang, Shenghu Zhang, Yuxiao Chen, Yue Qin, Junyang Li, Ziwei Zhai, Pengli Li, et al.supernatant was discarded, and then pellets were resuspended in proteinase K (Thermo Fisher Scientific, AM2548) and SDS for reverse-crosslinking by shaking for 2 h at 65 °C. The DNAs were extracted using DNA Clean

(11) Bidirectional CRISPR screens decode a GLIS3-dependent fibrotic cell circuitNatureJanuary 7, 2026Vladislav Pokatayev, Alok Jaiswal, Angela R. Shih, Åsa Segerstolpe, Bihua Li, Elizabeth A. Creasey, Yanhua Zhao, Crystal Lin, Shane Murphy, Chih-Hung Chou, et al.catalogue no. 19101) was added for 1 h at 65 °C; then, 0.4 mg ml−1 proteinase K (Thermo Fisher Scientific, AM2548) was added to digest proteins for 4 h at 65 °C. DNA was then extracted with phenol/chloroform/isoamyl

(12) Immune Cell Mitochondrial Phenotypes Are Largely Preserved in Mitochondrial Diseases and Do Not Reflect Disease SeverityNeurol GenetJanuary 2, 2026Cynthia C. Liu, Mangesh Kurade, Anna S. Monzel, Catherine Kelly, Robert-Paul Juster, Caroline Trumpff, Michio Hirano, Martin Picard[Sigma #P1379], 114 mM Tris-HCl pH 8.5 [Sigma #T3253], and 200 µg/mL of proteinase K [Thermo Fisher #AM2548] for 16 hours at 55°C), followed by proteinase K inactivation at 95°C for 10 minutes. qPCRs were performed

(13) Multidimensional mapping of stimulation-responsive regulatory elements and candidate causal variants in T cell activationSci AdvJanuary 1, 2026Zhao Wang, Wei Dong, Xiangling Feng, Xinran Xu, Yao An, Huiling Xiao, Menghan Luo, Qian Liang, Ruoxuan Bai, Ke Zhao, et al.after enzyme inactivation. Cross-links were reversed by adding Protein K (Thermo Fisher Scientific, AM2548) overnight at 65°C, followed by RNase A treatment for 45 min at 37°C. The ligation products were purified

(14) Single-cell RNA sequencing using split-pool barcoding reveals transcriptional heterogeneity in Porphyromonas gingivalis with implications for periodontal pathogenesisJournal of Oral MicrobiologyDecember 31, 2025Eun-Young Jang, Seok Bin Yang, Jeewan Chun, Kyu Hwan Kwack, Sang-Wook Kang, Jae-Hyung Lee, Ji-Hoi Moon50 mM EDTA, 400 mM NaCl (AM9759, Invitrogen) and 1% Triton X-100]. Proteinase K (5 μL of 20 mg/mL; AM2548, Invitrogen) was then added to the mixture. Cell lysis was performed by incubating the samples at 55°C

(15) Potent and durable gene modulation in heart and muscle with chemically defined lipophilic siRNAsNucleic Acids ResDecember 11, 2025Hassan H Fakih, Clemens Lochmann, Rosemary Gagnon, Ashley Summers, Jillian Caiazzi, Julianna E Buchwald, Qi Tang, Bruktawit Maru, Samuel R Hildebrand, Mohammad Zain U I Abideen, et al.consisting of one part lysis mixture, two parts deionized H2O and 0.167 mg/ml proteinase K (Invitrogen, #AM2548). Cell lysates were thoroughly mixed by pipetting up and down 15–20 times. Probe set validation was

(16) Histone Acetyltransferases Gcn5 and Esa1 Regulate Occupancy of RSC to Maintain Nucleosome-Depleted Regions and Promote RSC Recruitment to Coding Regions Genome-Wide in Saccharomyces cerevisiaeMolecular and Cellular BiologyDecember 2, 2025Emily Biernat, Mansi Verma, Matthew Werick, Uzair Khan, Sama Joseph, Chhabi K. Govindfor reverse crosslinking. Samples were then treated with 5 μl of proteinase K (20mg/ml, Ambion, cat# AM2548) for 2 hours and DNA was extracted twice using chloroform:isoamyl alcohol (IAA). The DNA was then ethanol

(17) Protamine lacunae preserve the paternal chromatin landscape in spermbioRxivNovember 21, 2025Thomas W Tullius, Rachel A Heuer, Stephanie C. Bohaczuk, Ben Mallory, Danilo Dubocanin, Jane Ranchalis, Ahmet Ayaz, Christopher E Mason, Emre Seli, Adam M Phillippy, et al.(Sigma Aldrich #70856) for 30 minutes at 37°C, and then with 6 μL of proteinase K (Thermo Fisher # AM2548) overnight at 55°C. The next morning, DNA was isolated using the ChIP DNA Clean & Concentrator kit (Zymo

(18) Molecular profiling and risk factors assessment of cutaneous leishmaniasis in an expanding endemic focus of Punjab, PakistanParasitology ResearchNovember 8, 2025Asma Ashraf, Saima Qadeer, Ume Amara Bukhari, Ismail Zeb, Akhtar Rasool, Umme Salma, Hadia Shaukat, Rehman Shahzad, Abdul Rahman Osmanicontaining 180 µL of tissue lysis buffer and 20 µL of proteinase K (20 mg/mL stock solution, Invitrogen, AM2548). The mixture was incubated at 56 °C overnight. A 200 µL aliquot from each lysate was then transferred

(19) The SP1-SuperEnhancer-SPHK1 Axis Mediates Niraparib Resistance in TNBCPharmaceuticals (Basel)October 27, 2025Yu-Xia Yuan, Rui-Jia Chen, Gui-Hui Tu, Chao-Qi Li, Long-Long Xu, Yi-Ling Lu, Li-Xian Wuby incubation with 200 mM NaCl at 65 °C for 6 h, followed by protease K digestion (Thermo Fisher, #AM2548). Purified DNA was analyzed by qPCR using SYBR Green Master Mix and primers flanking the SE region (primer

(20) Electron microscopy visualization of cell-free mitochondrial DNA–containing extracellular vesicles in human plasma, serum, and salivamedRxivOctober 22, 2025Alexandra Volos, Soah Grace Franklin, Jeremy Michelson, Shannon Rausser, Jonathan R. Brestoff, Martin PicardTween-20 [Sigma #P1379], 114 mM Tris-HCl pH 8.5 [Sigma #T3253], and 200μg/mL Proteinase K [ThermoFisher #AM2548]). After sealing and mixing the plate, the sample/lysis buffer mixture was then incubated overnight

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Recombinant Proteinase K Solution (20 mg/mL) from Thermo Fisher Scientific

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Recombinant Proteinase K Solution (20 mg/mL) from Thermo Fisher Scientific

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