Proteinase K Powder AM2544 from Thermo Fisher Scientific

Product Specs
ItemProteinase K Powder
CompanyThermo Fisher Scientific
Price Inquire
Catalog NumberAM2544
Size500 mg
TypeProteinase K
HostT. album

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Thermo Fisher Scientific

168 Third Avenue

Waltham, MA 02451

United States

Phone: 800-678-5599

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Website: www.thermofisher.com

(1) Functional Mapping of Epigenomic Regulators Uncovers Coordinated Tumor Suppression by the HBO1 and MLL1 ComplexesCancer DiscoveryDecember 2, 2025Yuning J. Tang, Haiqing Xu, Nicholas W. Hughes, Paloma Ruiz, Samuel H. Kim, Emily G. Shuldiner, Steven S. Lopez, Jess D. Hebert, Saswati Karmakar, Laura Andrejka, et al.(100mM NaCl, 20mM Tris, 10mM EDTA, 0.5% SDS) with 200 μl of 20 mg/ml Proteinase K (Life Technologies, AM2544). Homogenized tissues were incubated at 55°C overnight. Genomic DNA was extracted with phenol-chloroform

(2) In vivo multiplexed modeling reveals diverse roles of the TBX2 subfamily and Egr1 in Kr as-driven lung adenocarcinomaGenes & DiseasesSeptember 3, 2025Athar Khalil, Trang Dinh, Meaghan Parks, Rebecca C. Obeng, Berkley Gryder, Adam Kresak, Yuxiang Wang, Jeff Maltas, Madeline Bedrock, Xiangzhen Wei, et al.mM Tris, 10 mM EDTA, 0.5% SDS) supplemented with 200 μL of 20 mg/mL proteinase K (Life Technologies, AM2544). The homogenized tissue was incubated at 55 °C overnight. Subsequently, DNA extraction was carried

(3) Efficient and multiplexed somatic genome editing with Cas12a miceNature Biomedical EngineeringMay 30, 2025Jess D. Hebert, Haiqing Xu, Yuning J. Tang, Paloma A. Ruiz, Colin R. Detrick, Jing Wang, Nicholas W. Hughes, Oscar Donosa, Vicky P. Siah, Laura Andrejka, et al.FastPrep-24 5G tissue homogenizer (116005500, MP Biomedicals) and digested overnight with proteinase K (AM2544, Life Technologies). To remove RNA, samples were incubated with RNase A prior to genomic DNA extraction

(4) End resection and telomere healing of DNA double-strand breaks during nematode programmed DNA eliminationNucleic Acids ResearchAugust 27, 2024Brandon Estrem, Richard E Davis, Jianbin Wangresuspended in 2 ml buffer G2 (Qiagen, catalog #1014636) with proteinase K (1 mg/ml) (Invitrogen, catalog #AM2544). The embryos were lysed with five strokes in a 7-ml metal dounce followed by a 2 h incubation at 37°C

(5) Rapid Dot-Blot Immunoassay for Detecting Multiple Salmonella enterica SerotypesJournal of Microbiology and BiotechnologyMarch 28, 2024Jeongik Cho, Heymin Song, Hyun C. Yoon, Hyunjin YoonThermo Fisher Scientific, USA) at 37°C for 20 min and subsequently with 5 μl protease K (20 mg/ml, #AM2544; Invitrogen) at 59°C for 20 min. Then, 5 μl cell lysate solution was spotted on a nitrocellulose membrane

(6) Defining the mammalian coactivation of hepatic 12-h clock and lipid metabolismCell ReportsMarch 8, 2022Huan Meng, Naomi M. Gonzales, Sung Yun Jung, Yue Lu, Nagireddy Putluri, Bokai Zhu, Clifford C. Dacso, David M. Lonard, Bert W. O’Malley11965092 "DMEM, no glucose",Thermo Fisher Scientific,Cat# 11966025 Proteinase K,Thermo Fisher Scientific,Cat# AM2544 0.45μM syringe filter,Thermo Fisher Scientific,Cat# 09-740-106 Critical commercial assays,Critical

(7) Serglycin-Deficiency Causes Reduced Weight Gain and Changed Intestinal Cytokine Responses in Mice Infected With Giardia intestinalisFrontiers in ImmunologyJuly 8, 2021Zhiqiang Li, Dimitra Peirasmaki, Staffan Svärd, Magnus Åbrinkat 95°C for 10 min. To digest the tail tissue and extract mouse genomic DNA Proteinase K (2 μg/ul, #AM2544, Ambion) was added and the samples were incubated overnight at 55°C. The crude extracts were then heated

(8) Targeted mutagenesis in human iPSCs using CRISPR genome-editing toolsMethodsJuly 1, 2021Yicheng Long, Thomas R. CechmM EDTA, 0.2% SDS, 200 mM NaCl, stored at room temperature) with 100 μg/mL Proteinase K (Invitrogen AM2544)Resuspend the cell pellet in 200 μl supplemented lysis buffer. Incubate at 55 °C for 30 min.Add 200

(9) Rapid and inexpensive preparation of genome-wide nucleosome footprints from model and non-model organismsSTAR ProtocolsJune 1, 2021Laura E. McKnight, Johnathan G. Crandall, Thomas B. Bailey, Orion G.B. Banks, Kona N. Orlandi, Vi N. Truong, Drake A. Donovan, Grace L. Waddell, Elizabeth T. Wiles, Scott D. Hansen, et al.,New England Biolabs,Cat# M0206S RNase A,Fisher BioReagents,Cat# BP25391 Proteinase K,Invitrogen,Cat# AM2544 Quick CIP (calf intestinal phosphatase),New England Biolabs,Cat# M0525L ,, Critical commercial assays,Critical

(10) Allele‐specific proximal promoter hypomethylation of the telomerase reverse transcriptase gene ( TERT ) associates with TERT expression in multiple cancersMolecular OncologyOctober 1, 2020Teisha J. Rowland, Andrew J. Bonham, Thomas R. CechProtein and RNA were removed through incubation with 100 mm Tris pH 6.5, 11 mm EDTA, 60 μg Proteinase K (AM2544; Life Technologies, Carlsbad, CA, USA), and 1 μg DNase‐free RNase (EN0531; Thermo Scientific) for 60

(11) The Chymase Mouse Mast Cell Protease-4 Regulates Intestinal Cytokine Expression in Mature Adult Mice Infected with Giardia intestinalisCellsApril 9, 2020Li Z, Peirasmaki D, Svärd S, Åbrink M.95 ◦ C for 10 min. To digest the tail tissue and extract mouse genomic DNA Proteinase K (2 µg/uL, #AM2544, Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA) was added and the samples were incubated overnight

(12) EZH2 Inhibition Sensitizes CARM1-High, Homologous Recombination Proficient Ovarian Cancers to PARP InhibitionCancer cellFebruary 10, 2020Karakashev S, Fukumoto T, Zhao B, Lin J, Wu S, Fatkhutdinov N, Park PH, Semenova G, Jean S, Cadungog MG, et al.,Thermo Fisher,Cat#11668019 DNase I,Sigma,Cat#4536282001 RNase A,Thermo Fisher,Cat#EN0531 Proteinase K,Thermo Fisher,Cat#AM2544 Dynabeads protein A,Thermo Fisher,Cat#10002D Dynabeads protein G,Thermo Fisher,Cat#10004D TRizol,Thermo Fisher

(13) Engineered Chromatin Remodeling Proteins for Precise Nucleosome PositioningCell ReportsNovember 19, 2019Drake A. Donovan, Johnathan G. Crandall, Orion G.B. Banks, Zena D. Jensvold, Vi Truong, Devin Dinwiddie, Laura E. McKnight, Jeffrey N. McKnightProtein G,Thermo Fisher Scientific,10004D RNase A,Fisher BioReagents,BP25391 Proteinase K,Invitrogen,AM2544 "Glycogen, RNA grade",Thermo Scientific,FERR0551 "Glycogen, molecular biology grade",Thermo Scientific,FERR0551 NEBuffer 2

(14) A quantitative and multiplexed approach to uncover the fitness landscape of tumor suppression in vivoNature methodsJuly 1, 2017Rogers ZN, McFarland CD, Winters IP, Naranjo S, Chuang CH, Petrov D, Winslow MM.(100mM NaCl, 20mM Tris, 10mM EDTA, 0.5% SDS) with 200 μl of 20 mg/ml Proteinase K (Life Technologies, AM2544). Homogenized tissue was incubated at 55°C overnight. To maintain accurate representation of all tumors,

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Proteinase K Powder from Thermo Fisher Scientific

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Proteinase K Powder from Thermo Fisher Scientific

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