Description
Remains active under various conditions
Proteinase K from the fungus
Engyodontium album is a nonspecific serine protease that is useful for general digestion of proteins.
- Over a wide pH range–optimal activity between 6.5 and 9.5
- Under denaturing conditions–e.g., in the presence of SDS or urea
- In the presence of metal chelating agents–e.g., EDTA
- At comparatively high temperatures–optimum digestion temperature is 65°C
- Protein Filter: P
ApplicationsRemoval of endogenous nucleases during the preparation of DNA and RNA; preparation of tissue sections for
in situ hybridization.
Performance and quality testingEndodeoxyribonuclease and exodeoxyribonuclease assays; liquid form tested for absence of RNase activity.
Unit definitionOne mAnson unit is described as that amount of enzyme that liberates 1µmole of Folin-positive amino acid within one minute at 37°C using hemoglobin as a substrate. ChIP-on-Chip, Chromatin Biology, Chromatin Immunoprecipitation (ChIP), DNA and RNA Purification and Analysis, DNA Extraction, General RNA Purification Reagents and Accessories, General gDNA Purification Reagents and Accessories, Genomic DNA Purification, RNA Extraction, RNAi, Epigenetics and Non-Coding RNA Research, Total RNA Isolation