Klenow Fragment 3'-->5' exo MBS9141654 from MyBioSource.com

Description

Klenow Fragment (3'--> 5' exo-) is an N-terminal truncation of DNA Polymerase I which retains polymerase activity, but has lost the 5'--> 3' exonuclease activity and has mutations (D355A, E357A) which abolish the 3'--> 5' exonuclease activity. Klenow Fragment (3'--> 5' exo-) is isolated from a recombinant source. It generates probes using random primers and shows moderate strand displacement activity.
It is applicable to random priming labeling, DNA sequencing by the Sanger dideoxy method, second strand cDNA synthesis and second strand synthesis in mutagenesis protocols

Product Specs
ItemKlenow Fragment 3'-->5' exo
CompanyMyBioSource.com
Price $145.00Supplier Page View Company Product Page
Catalog NumberMBS9141654
Format25 mM Tris-HCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, pH 7.4 at 25 degree C
ApplicationsA-tailing, Polymerases for DNA Manipulation, DNA Sequencing, RT-PCR & cDNA Synthesis, PCR
Size200 Units
Molecular WeightTheoretical 68000 daltons
Concentration5,000 U/ml
Molecule NameKlenow Fragment 3'-->5' exo
Source/Expression SystemE Coli