Description
TREM2, also known as Triggering receptor expressed on myeloid cells 2 isoform 1, is a membrane protein that forms a receptorsignaling complex with the TYRO protein tyrosine kinase binding protein. This protein is found in immune cells termed myeloid cells, which include macrophages, granulocytes, monocytes, and dendritic cells. It functions in immune response and maybe involved in chronic inflammation by triggering the production ofconstitutive inflammatory cytokines. It also promotes the differentiation and function of osteoclasts, the production of inflammatory cytokines by adipocytes, insulin resistance, and the phagocytic clearance of bacteria. Defects in TREM2 are acause of polycystic lipomembranous osteodysplasia with sclerosingleukoencephalopathy (PLOSL). In addition, TREM2 is overexpressed in many tumor types and has anti-inflammatory activities. It might therefore be a good therapeutic target. Recombinant human TREM2, fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques