Description
NKp80/KLRF1, also known as killer cell lectin-like receptor subfamily F member 1, is a member of the C-type lectin family. Multiple splicing variants produce four isoforms for NKp80/KLRF1 and isoform 1 is the standard protein. It is expressed on NK cells and a subset of T lymphocytes whereas it is not expressed on B lymphocytes, monocytes and granulocytes. It stimulates NK cell cytotoxicity and induces calcium influx after triggering by appropriate antibodies. It works through interaction with its ligand, AICL (activation-induced C-type lectin), which is selectively expressed on myeloid cells. NKp80-AICL interaction regulates the immune responses at sites of inflammation by stimulating the release of proinflammatory cytokines. Recombinant human NKp80/KLRF1, fused to hIgG-His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques