Description
ALCAM (Activated leukocyte cell adhesion molecule), also known as CD166 antigen, is a member of the Ig CAM family within the immunoglobulin superfamily. It interacts with CD6 that promotes T-cell activation and proliferation and plays a role in the binding of T- and B-cells to activated leukocytes. While expressed in a wide variety of tissues, ALCAM is expressed on activated T cells, activated monocytes, epithelial cells, fibroblasts, neurons, melanoma cells, and also in sweat and sebaceous glands. Recently, CD166 is regarded as a potential novel breast cancer indicator and therapeutic target. Recombinant mouse ALCAM, fused to hIgG-His-tag at C-terminus, was expressed in Insect cell and purified by using conventional chromatography techniques