Description
Short Description: Recombinant Mononucleosomes H3.3 (G34R)-biotinylated consist of a 167 bp of 601 DNA with 5' biotin tag and two molecules each of histones H2A that includes amino acids 1-130 (end)(accession number NP_003503.1), H2B that includes amino acids 1-126 (end)(accession number NP_003509.1), H3.3 that includes amino acids 1-136 (end)(accession number NP_002098.1) with a point mutation Gly34Arg, and H4 that includes amino acids 1-103 (end)(accession number NP_003539.1). All of these histones were expressed in E Coli. The molecular weight of histone octamer is ~108 kDa. The recombinant protein is >=95% by SDS-PAGE gel. Nucleosomes comprise the smallest subunit of chromatin; they consist of 147 bp of DNA wrapped around an octamer of core histone proteins (H2A, H2B, H3 and H4). Therefore, nucleosomes are more physiologically relevant substrates than histones and histone-derived peptides for use in in vitro studies. More importantly, some histone methyltransferases are significantly more active, as well as specific, when using nucleosomal substrates in HMT assays, such as DOT1L and NSD family enzymes. Nucleosomes are also widely used in histone methyltransferase screening assays to identify small molecular inhibitors for drug discovery