Description
A DNA sequence encoding the extracellular domain (Met 1-Ser 298) of rat FCGRT (P13599) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the rat B2M (P07151) (Met 1-Met 119) constructed the plasmid 2. The two plasmids were co-expressed and the FCGRT/B2M heterodimer was purified