Description
Short Description: Recombinant Mononucleosomes H3.1 (R26C)-biotin consist of a 167 bp of 601 DNA with 5' biotin tag and two molecules each of histones H2A that includes amino acids 1-130 (end)(accession number NM_003512), H2B that includes amino acids 1-126 (end)(accession number NM_003518), H3.1 that includes amino acids 1-136 (end)(accession number NM_003529) with a point mutation Arg26Cys, and H4 that includes amino acids 1-103 (end)(accession number NM_003548). All of these histones were expressed in E Coli cells. The molecular weight of histone the octamer is 108 kDa. Recombinant Mononucleosomes H3.1 (R26C)-biotin is suitable for use as substrate for histone modification enzymes, or to generate chromatin in vitro