Description
Rat Complement C5 Recombinant Protein His Tag Lyophilized has been recombinantly produced in HEK293 cells.
Derived from proteolytic degradation of complement C5, C5 anaphylatoxin is a mediator of local inflammatory process. C5 precursor is first processed by the removal of 4 basic residues, forming two chains, beta and alpha, linked by a disulfide bond. C5 convertase activates C5 by cleaving the alpha chain, releasing C5a anaphylatoxin and generating C5b (beta chain + alpha' chain). Activation of C5 by a C5 convertase initiates the spontaneous assembly of the late complement components, C5-C9, into the membrane attack complex. C5b has a transient binding site for C6. The C5b-C6 complex is the foundation upon which the lytic complex is assembled. The C5a anaphylatoxin interacts with C5AR1 and tick complement inhibitor. C5a is also a potent chemokine which stimulates the locomotion of polymorphonuclear leukocytes and directs their migration toward sites of inflammation.
This protein carries a polyhistidine Tag at the C-terminal. The mature form of Complement C5 is a disulfide-linked heterodimer composed of proteolytically cleaved alpha and beta chain. Each alpha and beta chain has a calculated MW of 74.1 kDa (beta chain) and 114.8 kDa (alpha chain)