Fig 1: nBMP2 is expressed in the nuclei of wild type but not mutant CA1 region hippocampal neurons.(A) Brain sections from wild type and nBmp2NLStm mice were stained with fluorescently tagged anti-BMP2 antibody (red) and nuclei were stained with DAPI (blue). The CA1 region of the hippocampus is shown. High magnification insets of the highlighted region illustrate red BMP2 labeling in the nuclei of pyramidal cell soma of the stratum pyramidale in wild type mice, which is absent in the nBmp2NLStm mice. BMP2 labeling of neuronal projections is present in both wild type and mutant mice, confirming that the targeted mutation selectively eliminated only the nuclear variant of the protein. (B) Brain sections from GAD67-GFP fusion knock-in mice were stained with fluorescently tagged anti-BMP2 antibody (red). All sections were imaged using laser confocal microscopy. Scale bars indicate 100 μm. SP: stratum pyramidale; SR: stratum radiatum.
Fig 2: nBMP2 is synthesized by translation from a downstream alternative start codon.The BMP2 preproprotein as it is initially translated into the ER is shown schematically with the ER signal peptide (light grey), the propeptide (dark grey), and the mature peptide (black). The downward arrow indicates the site at which the BMP2 proprotein undergoes proteolytic cleavage by proprotein convertases in the Golgi to release the mature secreted peptide. Initiation at codon 53 (marked) results in cytoplasmic rather than ER translation because the ER signal peptide is absent. The protein therefore avoids proteolytic cleave and is directed to the nucleus by the bipartite nuclear localization signal (NLS) (key amino acids are in bold). The three amino acids (RKR) that were changed to alanines (AAA) to prevent nuclear localization of nBMP2 in the nBmp2NLStm mouse are shown.
Supplier Page from Novus Biologicals, a Bio-Techne Brand for BMP-2 Peptide