LAP3 (NM_015907) Human Recombinant Protein TP309052L from OriGene Technologies

Product Specs
ItemLAP3 (NM_015907) Human Recombinant Protein
CompanyOriGene Technologies
Price $7,820.00Supplier Page View Company Product Page
Catalog NumberTP309052L
TypeRecombinant Proteins
Molecular Weight56 kDa
Conjugate/TagC-Myc/DDK
Size1 mg
SpeciesHuman
NCBI Full Gene Nameleucine aminopeptidase 3
NCBI Gene AliasesHEL-S-106, LAP, LAPEP, PEPS
Molecule NameLAP3
Source/Expression SystemHEK293T
Format25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
Concentration>0.05 µg/µL as determined by microplate BCA method
Purity> 80% as determined by SDS-PAGE and Coomassie blue staining
ActivityLAP3 activity verified in a biochemical assay: Leucine aminopeptidase 3 (LAP3, TP309052) activity was measured in a fluorescent biochemical assay. LAP3 catalyzes the removal of unsubstituted N-terminal amino acids from various peptides and is most active on leucine. LAP3 activity was measured in a 100 µl reaction mixture containing 1 mM L-leucine 7-amido-4-methyl coumarin (Leu-AMC), 50 mM Tris, pH 8.0, 4 mM MgCl2, and 1 mM MnCl2. Cleavage of leucine from the AMC moiety results in a strong increase in fluorescence intensity. Fluorescence was measured over time with an excitation wavelength of 380 nm and an emission wavelength of 460 nm. The activity of the enzyme in this system remained constant over six hours