GBA (NM_000157) Human Recombinant Protein TP316061L from OriGene Technologies

Product Specs
ItemGBA (NM_000157) Human Recombinant Protein
CompanyOriGene Technologies
Price $9,200.00Supplier Page View Company Product Page
Catalog NumberTP316061L
TypeRecombinant Proteins
Molecular Weight55.5 kDa
Conjugate/TagC-Myc/DDK
Size1 mg
SpeciesHuman
NCBI Full Gene Nameglucosylceramidase beta 1
NCBI Gene AliasesGBA, GCB, GLUC
Molecule NameGBA
Source/Expression SystemHEK293T
Format25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
Concentration>0.05 µg/µL as determined by microplate BCA method
Purity> 80% as determined by SDS-PAGE and Coomassie blue staining
ActivityThe enzymatic activity of TP316061 (GBA) was measured by its ability to hydrolyze a fluorescent substrate 4-methylumbelliferyl-β-D-glucopyranoside. The specific activity is > 70,000 pmol/hour/µg, as measured under the following conditions: 27 ng of GBA was incubated with 10 mM 4-methylumbelliferyl- β-D-glucopyranoside in the following buffer at 37°C for 40 min: 150 mM citrate-phosphate buffer, pH 5.4, 0.25% (w/w) sodium taurocholate, 0.25% (w/w) Triton X-100, and 1% bovine serum albumin. The reaction was terminated by adding 0.5 volume of 1M glycine buffer, pH 12.5. The hydrolyzed product of reaction, 4-methylumbelliferone (4-MU), was measured using a FlexStation 3 microplate reader (Ex365/Em445). Specific activity of GBA was calculated based on a standard curve of known concentration of 4-MU