CSK (NM_004383) Human Recombinant Protein TP310758L from OriGene Technologies

Product Specs
ItemCSK (NM_004383) Human Recombinant Protein
CompanyOriGene Technologies
Price $7,820.00Supplier Page View Company Product Page
Catalog NumberTP310758L
TypeRecombinant Proteins
Molecular Weight50.5 kDa
Conjugate/TagC-Myc/DDK
Size1 mg
SpeciesHuman
NCBI Full Gene NameC-terminal Src kinase
Molecule NameCSK
Source/Expression SystemHEK293T
Format25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
Concentration>0.05 µg/µL as determined by microplate BCA method
Purity> 80% as determined by SDS-PAGE and Coomassie blue staining
ActivityCSK activity verified in a biochemical assay: CSK (c-src tyrosine kinase) (TP310758) activity was measured in a homogeneous time-resolved fluorescent (HTRF®) assay. CSK is a tyrosine kinase known to phosphorylate LCK, FYN and LYN. Varying concentrations of CSK were added to a reaction mix containing ATP and a biotinylated kinase substrate and the reaction mixture was incubated to allow the protein to phosphorylate the tyrosine residue in the substrate. HTRF detection reagents were then added, and the time-resolved fluorescent signal was measured on a Flexstation 3 microplate reader. The time resolved fluorescent signal is expressed as “delta R” or “ΔR” and is a ratio calculated from the fluorescent emission intensities of the donor and acceptor fluors