FYN (NM_002037) Human Recombinant Protein TP324691L from OriGene Technologies

Product Specs
ItemFYN (NM_002037) Human Recombinant Protein
CompanyOriGene Technologies
Price $7,820.00Supplier Page View Company Product Page
Catalog NumberTP324691L
TypeRecombinant Proteins
Molecular Weight60.6 kDa
Conjugate/TagC-Myc/DDK
Size1 mg
SpeciesHuman
NCBI Full Gene NameFYN proto-oncogene, Src family tyrosine kinase
NCBI Gene AliasesSLK, SYN, p59-FYN
Molecule NameFYN
Source/Expression SystemHEK293T
Format25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
Concentration>0.05 µg/µL as determined by microplate BCA method
Purity> 80% as determined by SDS-PAGE and Coomassie blue staining
ActivityFYN activity verified in a biochemical assay: FYN (FYN oncogene related to SRC, FGR, YES) (TP324691) activity was measured in a homogeneous time-resolved fluorescent (HTRF®) assay. FYN is a non-receptor tyrosine kinase and is a member of the Src kinase family consisting of Src, Fyn, Yes, Lck, Lyn, Hck, Fgr and Blk. Varying concentrations of FYN were added to a reaction mix containing ATP and a biotinylated kinase substrate and the reaction mixture was incubated to allow the protein to phosphorylate the substrate. HTRF detection reagents were then added, and the time-resolved fluorescent signal was measured on a Flexstation 3 microplate reader. The time resolved fluorescent signal is expressed as “delta R” or “&DeltaR” and is a ratio calculated from the fluorescent emission intensities of the donor and acceptor fluors