SMAD3 (NM_005902) Human Recombinant Protein TP308749M from OriGene Technologies

Product Specs
ItemSMAD3 (NM_005902) Human Recombinant Protein
CompanyOriGene Technologies
Price $2,950.00Supplier Page View Company Product Page
Catalog NumberTP308749M
Size100 µg
Conjugate/TagC-Myc/DDK
Format25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
Concentration>0.1 µg/µL as determined by microplate BCA method
ActivitySMAD3 Activity Verified in a DNA-binding Assay: SMAD3 (TP308749) activity was measured in a colorimetric DNA-binding assay. Purified SMAD3 protein containing a C-terminal MYC/DDK tag was incubated with biotinylated double-stranded oligonucleotide containing the SMAD3 consensus DNA-binding sequence. Following incubation, the reaction was transferred to a streptavidin-coated microplate to allow capture of the DNA-protein complex. After washing, the captured protein was detected with an anti-DDK peroxidase conjugate and colorimetric signal detection with TMB. Specificity of the protein-DNA interaction was confirmed by carrying out the binding in the presence of an unlabeled competitor oligonucleotide and by comparison to binding to an oligonucleotide containing a mutation in the consensus binding sequence
TypeRecombinant Proteins
Molecular Weight47.9 kDa
SpeciesHuman
Molecule NameSMAD3
Source/Expression SystemHEK293T
Purity> 80% as determined by SDS-PAGE and Coomassie blue staining
NCBI Full Gene NameSMAD family member 3
NCBI Gene AliasesHSPC193, HsT17436, JV15-2, LDS1C, LDS3, MADH3, hMAD-3, hSMAD3, mad3