IDO1 (NM_002164) Human Recombinant Protein TP306592M from OriGene Technologies

Product Specs
ItemIDO1 (NM_002164) Human Recombinant Protein
CompanyOriGene Technologies
Price $2,508.00Supplier Page View Company Product Page
Catalog NumberTP306592M
TypeRecombinant Proteins
Molecular Weight45.1 kDa
Conjugate/TagC-Myc/DDK
Size100 µg
SpeciesHuman
NCBI Full Gene Nameindoleamine 2,3-dioxygenase 1
NCBI Gene AliasesIDO, IDO-1, INDO
Molecule NameIDO1
Source/Expression SystemHEK293T
Format25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
Concentration>0.05 µg/µL as determined by microplate BCA method
Purity> 80% as determined by SDS-PAGE and Coomassie blue staining
ActivityThe specific activity of IDOI was determined by monitoring kynurenine formation from N-formylkynurenine based on the absorbance at 492nm. The N-formylkynurenine was produced from a conversion of tryptophan with IDO1. The reaction was carried out at 25°C for 15min in the buffer containing 100mM PBS, pH6.5, 40mM ascorbic acid, 450 units catalase, 20µM methylene blue, and 800µM L-tryptophan as the substrate. The reaction was terminated by adding 50ul of 30% (w/v) trichloroacetic acid. The sample was further incubated for 30min at 60°C and centrifuged at 12000 rpm for 15 min. The supernatant was used to mix with an equal volume of Ehrlich’s reagent (2% p-dimethylaminobenzaldehyde in glacial acetic acid) to measure the absorbance at 492 nm after 10min incubation