CSRP3 is the substrate of O-glycosidase, according to Western blot (WB) and Amaranthus caudatus lectin blot (LB) analyses of recombinant human CSRP3. Recombinant proteins expressed in E. coli (unglycosylated negative control) and HEK293 cells (potentially glycosylated reference) (OriGene TP325217) were analyzed after treatment with sialidase and O-glycosidase. Figure cited from PLoS ONE, PMID: 27281159
CSRP3 binds to Amaranthus caudatus lectin (ACA), according to Western blot (WB) and ACA lectin blot (LB) analyses of recombinant human CSRP3. Recombinant proteins expressed in E. coli (unglycosylated negative control) and HEK293 cells (potentially glycosylated reference) (OriGene TP325217) were analyzed after treatment with sialidase and O-glycosidase. Figure cited from PLoS ONE, PMID: 27281159
Altered O-glycosylation on CSRP3 in the left ventricle of Dahl salt-sensitive rats, according to Western blot (WB) and Amaranthus caudatus lectin blot (LB) analyses of CSRP3 from the left ventricle extracts of Dahl salt-sensitive rats under a low-salt (LS) or high-salt (HS) diet for 12 or 16 weeks. CSRP3 in the extracts was immunoprecipitated, denatured, separated by SDS-PAGE, and analyzed. Recombinant human CSRP3 (OriGene TP325217) served as a control for immunoprecipitation with the anti-CSRP3 antibody (+) or normal IgG (-). Figure cited from PLoS ONE, PMID: 27281159
Coomassie blue staining of purified CSRP3 protein (Cat# TP325217). The protein was produced from HEK293T cells transfected with CSRP3 cDNA clone (Cat# RC225217) using MegaTran 2.0 (Cat# TT210002).
Supplier Page from OriGene Technologies for CSRP3 (NM_001127656) Human Recombinant Protein