B Raf (BRAF) (NM_004333) Human Recombinant Protein TP311013M from OriGene Technologies | Biocompare.com

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B Raf (BRAF) (NM_004333) Human Recombinant Protein
B Raf (BRAF) (NM_004333) Human Recombinant Protein

Coomassie blue staining of purified BRAF protein (Cat# TP311013). The protein was produced from HEK293T cells transfected with BRAF cDNA clone (Cat# RC211013) using MegaTran 2.0 (Cat# TT210002).

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B Raf (BRAF) (NM_004333) Human Recombinant Protein

Description

Recombinant protein of human v-raf murine sarcoma viral oncogene homolog B1 (BRAF), 100 µg

Product Specs
ItemB Raf (BRAF) (NM_004333) Human Recombinant Protein
CompanyOriGene Technologies
Price $2,950.00Supplier Page View Company Product Page
Catalog NumberTP311013M
TypeRecombinant Proteins
Molecular Weight84.3 kDa
Conjugate/TagC-Myc/DDK
Size100 µg
SpeciesHuman
NCBI Full Gene NameB-Raf proto-oncogene, serine/threonine kinase
NCBI Gene AliasesB-RAF1, B-raf, BRAF-1, BRAF1, NS7, RAFB1
Molecule NameB Raf
Source/Expression SystemHEK293T
Format25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
Concentration>0.1 µg/µL as determined by microplate BCA method
Purity> 80% as determined by SDS-PAGE and Coomassie blue staining
ActivityBRAF kinase activity was measured in an HTRF® assay. Varying concentrations of BRAF were added to a reaction mix containing ATP and a biotinylated kinase substrate (HTRF substrate 2) and was incubated at 37C for phosphorylation. HTRF detection reagents were then added, the reaction was incubated for 30 minutes at room temperature. Time-resolved fluorescent signal (Delta R) was measured on a Flexstation 3 microplate reader

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OriGene Technologies

OriGene Technologies

9620 Medical Center Drive # 200

Rockville, Maryland 20850

United States

Phone: 1-888-267-4436 (U.S. only) / 301-340-3188
301-340-9254

Company Profile

Website: http://www.origene.com

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