ETS1 (NM_005238) Human Recombinant Protein TP315203M from OriGene Technologies

Product Specs
ItemETS1 (NM_005238) Human Recombinant Protein
CompanyOriGene Technologies
Price $2,508.00Supplier Page View Company Product Page
Catalog NumberTP315203M
TypeRecombinant Proteins
Molecular Weight50.2 kDa
Conjugate/TagC-Myc/DDK
Size100 µg
SpeciesHuman
NCBI Full Gene NameETS proto-oncogene 1, transcription factor
NCBI Gene AliasesETS-1, EWSR2, c-ets-1, p54
Molecule NameETS1
Source/Expression SystemHEK293T
Format25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
Concentration>0.05 µg/µL as determined by microplate BCA method
Purity> 80% as determined by SDS-PAGE and Coomassie blue staining
ActivityETS1 Activity Verified in a DNA-binding Assay: ETS1 (TP315203) activity was measured in a colorimetric DNA-binding assay. Purified ETS1 protein containing a C-terminal MYC/DDK tag was incubated with biotinylated double-stranded oligonucleotide containing the ETS1 consensus DNA-binding sequence. Following incubation, the reaction was transferred to a streptavidin-coated microplate to allow capture of the protein-DNA complex. After washing, the captured protein was detected with an anti-DDK peroxidase conjugate and colorimetric signal detection with TMB. Specificity of the protein-DNA interaction was confirmed by carrying out the binding in the presence of an unlabeled competitor oligonucleotide and by comparison to binding to an oligonucleotide containing a mutation in the consensus binding sequence