ALK (NM_004304) Human Recombinant Protein TP322485M from OriGene Technologies

Product Specs
ItemALK (NM_004304) Human Recombinant Protein
CompanyOriGene Technologies
Price $2,950.00Supplier Page View Company Product Page
Catalog NumberTP322485M
TypeRecombinant Proteins
Molecular Weight176.3 kDa
Conjugate/TagC-Myc/DDK
Size100 µg
SpeciesHuman
NCBI Full Gene NameALK receptor tyrosine kinase
NCBI Gene AliasesALK1, CD246, NBLST3
Molecule NameALK
Source/Expression SystemHEK293T
Format25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
Concentration>0.05 µg/µL as determined by microplate BCA method
Purity> 80% as determined by SDS-PAGE and Coomassie blue staining
ActivityALK activity verified in a biochemical assay: ALK (anaplastic lymphoma receptor tyrosine kinase) (TP322485) activity was measured in a homogeneous time-resolved fluorescent (HTRF®) assay. ALK is an orphan receptor protein-tyrosine kinase having a putative transmembrane domain and an extracellular domain. Varying concentrations of ALK were added to a reaction mix containing ATP and a biotinylated kinase substrate and the reaction mixture was incubated to allow the protein to phosphorylate the substrate. HTRF detection reagents were then added, and the time-resolved fluorescent signal was measured on a Flexstation 3 microplate reader. The time resolved fluorescent signal is expressed as “delta R” or “ΔR” and is a ratio calculated from the fluorescent emission intensities of the donor and acceptor fluors