FGR (NM_005248) Human Recombinant Protein TP314458L from OriGene Technologies

Product Specs
ItemFGR (NM_005248) Human Recombinant Protein
CompanyOriGene Technologies
Price $9,200.00Supplier Page View Company Product Page
Catalog NumberTP314458L
TypeRecombinant Proteins
Molecular Weight59.3 kDa
Conjugate/TagC-Myc/DDK
Size1 mg
SpeciesHuman
NCBI Full Gene NameFGR proto-oncogene, Src family tyrosine kinase
NCBI Gene AliasesSRC2, c-fgr, c-src2, p55-Fgr, p55c-fgr, p58-Fgr, p58c-fgr
Molecule NameFGR
Source/Expression SystemHEK293T
Format25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol
Concentration>0.05 µg/µL as determined by microplate BCA method
Purity> 80% as determined by SDS-PAGE and Coomassie blue staining
ActivityFGR activity verified in a biochemical assay: FGR (Gardner-Rasheed feline sarcoma viral (v-fgr) oncogene homolog) (TP314458) activity was measured in a homogeneous time-resolved fluorescent (HTRF®) assay. FGR is a tyrosine kinase that is a member of the Src family of protein tyrosine kinases. Varying concentrations of FGR were added to a reaction mix containing ATP and a biotinylated kinase substrate and the reaction mixture was incubated to allow the protein to phosphorylate the substrate. HTRF detection reagents were then added, and the time-resolved fluorescent signal was measured on a Flexstation 3 microplate reader. The time resolved fluorescent signal is expressed as “delta R” or “ΔR” and is a ratio calculated from the fluorescent emission intensities of the donor and acceptor fluors