Processing of pre-miR-130b by recombinant Dicer and AGO2 proteins in vitro. Twenty pmol of pre-miR-130b was incubated with 2 U of Dicer or 200 ng of Ago2 (OriGene TP328592) for four hours. The processed products were detected using Northern blotting with a biotin-labeled anti-miR-130b probe. Lane 1: 22 nt biotin-ssRNA; 2: pre-miR-130b; 3: pre-miR-130b with Dicer; 4: pre-miR-130b with AGO2. Figure cited from Sci Rep, PMID: 28769054
Coomassie blue staining of purified AGO2 protein (Cat# TP328592). The protein was produced from HEK293T cells transfected with AGO2 cDNA clone (Cat# RC228592) using MegaTran 2.0 (Cat# TT210002).
Supplier Page from OriGene Technologies for Argonaute 2 (AGO2) (NM_001164623) Human Recombinant Protein