Human TROP2 Protein (ECD, His & AVI Tag), Biotinylated 10428-H49H-B from Sino Biological, Inc.

Product Specs
ItemHuman TROP2 Protein (ECD, His & AVI Tag), Biotinylated
CompanySino Biological, Inc.
Price $298.00Supplier Page View Company Product Page
Catalog Number10428-H49H-B
TypeRecombinant protein
Molecule NameTACSTD2
SpeciesHuman
NCBI Full Gene Nametumor associated calcium signal transducer 2
NCBI Gene AliasesEGP-1, EGP1, GA733-1, GA7331, GP50, M1S1, TROP2
Conjugate/TagC-His-AVI
FormatLyophilized from sterile 25mM Sodium Citrate, 200mM NaCl, 0.02%tween80, 10% Trehalose, pH 6.0
Source/Expression SystemHEK293 Cells
Molecular WeightThe recombinant human TACSTD2 consists of 274 amino acids and predicts a molecular mass of 31.14 kDa. It migrates as an approximately 48.07 kDa band in SDS-PAGE under reducing conditions
Purity> 95 % as determined by SDS-PAGE. > 90 % as determined by SEC-HPLC
Activity1.Loaded Anti-TROP2 (Research Grade Sacituzumab Biosimilar) on ProA Biosensor, can bind Recombinant Human TROP2 Protein, His Tag (Cat. No. 10428-H49H-B) with an affinity constant of 0.373nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested). 2.Immobilized Anti-TROP2 (Research Grade Sacituzumab Biosimilar) at 2µg/mL (100µL/well) can bind Recombinant Human TROP2 Protein (ECD, His & AVI Tag), Biotinylated (Cat: 10428-H49H-B), the EC50 is 0.8-2.4 ng/mL(Routinely tested). 3.Anti-TROP2 (Research Grade Datopotamab Biosimilar) captured on CM5 chip via Anti-Human IgG Fc, can bind Recombinant Human TROP2 Protein (ECD, His & AVI Tag), Biotinylated (Cat: 10428-H49H-B) with an affinity constant of 16.900 nM as determined in an SPR assay (Biacore 8K) (Routinely tested). 4.Immobilized Recombinant Human TROP2 Protein (ECD, His & AVI Tag), Biotinylated (Cat: 10428-H49H-B) at 1 µg/mL (100 µl/well) on streptavidin precoated (5 µg/mL, 100 µL/well) can bind Anti-TROP2 (Research Grade Datopotamab Biosimilar), the EC50 is 0.6-2.0 ng/mL (QC tested)
Size20 µg