Human CD32A Protein (AVI & His Tag), Biotinylated 10374-H27H2-B from Sino Biological, Inc.

Description

A DNA sequence encoding the extracellular domain (Ala36-Met210) of human FCGR2A (P12318-1, with Natural variant H167) was fused with a C-terminal polyhistidine tagged AVI tag at the C-terminus. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed

Product Specs
ItemHuman CD32A Protein (AVI & His Tag), Biotinylated
CompanySino Biological, Inc.
Price $688.00Supplier Page View Company Product Page
Catalog Number10374-H27H2-B
TypeRecombinant protein
Molecule NameFCGR2A
SpeciesHuman
Conjugate/TagC-AVI, His
FormatLyophilized from sterile PBS, pH 7.4
Source/Expression SystemHEK293 Cells
Molecular WeightThe recombinant human FCGR2A consists of 207 amino acids and predicts a molecular mass of 23.21 kDa. It migrates as an approximately 32.14 kDa band in SDS-PAGE under reducing conditions
Purity> 95 % as determined by SDS-PAGE. > 95 % as determined by SEC-HPLC
ActivityLoaded Recombinant Human CD32A Protein (H167,AVI & His Tag), Biotinylated (Cat: 10374-H27H2-B) on SA Biosensor, can bind anti-CD20(Research grade Rituximab Biosimilar) with an affinity constant of 0.51 µM as determined in BLI assay (Sartorius Octet RED384) (QC tested)
Size100 µg