PTMScan ® Control Peptides Phospho-Akt (RXXS*/T*) 62248S from Cell Signaling Technology

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ItemPTMScan ® Control Peptides Phospho-Akt (RXXS*/T*)
CompanyCell Signaling Technology
Price $232.00 Inquire
Catalog Number62248S
Size100 µl
ApplicationsPTMScan

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Cell Signaling Technology

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Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

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Website: http://www.cellsignal.com/

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(1) The ASFV CD2v protein inhibits apoptosis by inducing proteasomal degradation of Bim EL via activation of the TPL2-MEK-ERK signaling pathwayJournal of VirologyFebruary 3, 2026Jianyu Zeng, Xin Zhang, Jingting Zhao, Qiang Li, Zhiyong Xiang, Fengyang Shi, Hua Wang, Wenlian Weng, Qiongqiong Zhou, Peng Gao, et al.peroxidase-conjugated goat anti-rabbit (ZB-2301) and anti-mouse (ZB-2305) IgGs were purchased from ZSGB-BIO. DAPI (62248) and Alexa Fluor 488-conjugated goat anti-mouse F(ab′)2 fragment (A11017) were obtained from Thermo Fisher Scientific

(2) OGFOD1 enables AML chemo- and nutrient stress resistance by regulating protein synthesisCell MetabolismOctober 7, 2025Christina Mayerhofer, Dan Li, Trine Kristiansen, Ernst Mayerhofer, Azeem Sharda, Giulia Schiroli, Karin Gustafsson, Lingli He, Michael Mazzola, Sam Keyes, et al.(7AAD),BD Biosciences,Cat#: 559925 "4’,6-diamidino-2-phenylindole (Dapi)",Thermo Fischer Scientific,Cat#: 62248 Anti-BCAT1 (rabbit monoclonal),Cell Signaling Technology,Cat#: CST88785 Anti-BCKDH E1α (rabbit monoclonal),Cell

(3) The inner nuclear membrane protein LEMD3 organizes the 3D chromatin architecture to maintain vascular smooth muscle cell identityNat CommunOctober 3, 2025Wenqiang Li, Yunxi Liao, Zhujiang Liu, Longjian Niu, Jiaqi Huang, Yiting Jia, Ran Xu, Sudun Guan, Zhenhui Liang, Yiran Li, et al.(BN10053) was purchased from Biorigin, Inc. (Beijing, China). 4,6-Diamidino-2-phenylindole (DAPI) (62248), Alexa Fluor 488-conjugated goat anti-rabbit IgG (A-11008, dilution 1:1000), and Alexa Fluor 555-conjugated

(4) Monocytes can efficiently replace all brain macrophages and fetal liver monocytes can generate bona fide SALL1+ microgliaImmunityMay 13, 2025Jonathan Bastos, Carleigh O'Brien, Mónica Vara-Pérez, Myrthe Mampay, Lynn van Olst, Liam Barry-Carroll, Daliya Kancheva, Sophia Leduc, Ayla Line Lievens, Leen Ali, et al.Cell Signaling) at room temperature for 2 hours. Nuclear counterstaining was performed using DAPI (1:5000, #62248, ThermoFisher) for 15 minutes. Slides were imaged using a PhenoImager HT (Akoya Biosciences) in slide

(5) KSHV hijacks the antiviral kinase IKKε to initiate lytic replicationPLoS pathogensJanuary 1, 2025Wang X, Liu Z, Xu X, Wang X, Ming Z, Liu C, Gao H, Li T, Liang Q.followed by a fluorescent secondary antibody for 1 h at room temperature. DAPI (Thermo Fisher Scientific, #62248) was used to mark nuclei. Primary antibodies used in this study included rabbit anti-Flag (Cell Signaling Technology

(6) Machine-guided design of cell-type-targeting cis-regulatory elementsNatureOctober 31, 2024Sager J. Gosai, Rodrigo I. Castro, Natalia Fuentes, John C. Butts, Kousuke Mouri, Michael Alasoadura, Susan Kales, Thanh Thanh L. Nguyen, Ramil R. Noche, Arya S. Rao, et al.further rinsed with PBS three times, followed by staining for nuclei with DAPI (Thermo Fisher Scientific, 62248). The sections were mounted onto slides with Prolong Gold antifade reagent (Cell Signalling Technology,

(7) Commensal microbiome dysbiosis elicits interleukin-8 signaling to drive fibrotic skin diseasePNAS NexusJuly 1, 2024Zhang W, Peng Q, Huang X, Huang Q, Zhang Z, Li F, Zheng N, Shi B, Fan Z, Maj T, et al.Cell Signaling Technology, 1:400). Cell nuclei were stained with 4,6-diamidino-2-phenylindole (DAPI, 62248, Thermo Scientific). Slides were scanned by a digital slide scanner, and fluorescent images were obtained

(8) Development of CD46 targeted alpha theranostics in prostate cancer using 134Ce/225Ac-Macropa-PEG4-YS5TheranosticsJanuary 27, 2024Kondapa Naidu Bobba, Anil P. Bidkar, Anju Wadhwa, Niranjan Meher, Suchi Drona, Alexandre M. Sorlin, Scott Bidlingmaier, Li Zhang, David M. Wilson, Emily Chan, et al.80312, 1:250 dilutions) antibodies; while nuclei were stained with DAPI (Thermo Scientific, Cat no. 62248, 1:1000 dilutions from 1 mg/mL). Secondary staining was carried out with an anti-rabbit secondary antibody

(9) mTOR Inhibitor Rapalink-1 Prevents Ethanol-Induced Senescence in Endothelial CellsCellsNovember 11, 2023Huakang Zhou, Xuanchen Li, Majeed Rana, Jan Frederick Cornelius, Dilaware Khan, Sajjad Muhammad1:100, Alexa Fluor 594, Cat. No.: 8889, Cell signaling, Danvers, MA, USA) for 1 h at RT. DAPI (cat no: 62248, Thermo Fisher Scientific, Waltham, MA, USA) was used for nuclear staining. The images were taken at

(10) Deep and fast label-free Dynamic Organellar MappingNature CommunicationsAugust 29, 2023Julia P. Schessner, Vincent Albrecht, Alexandra K. Davies, Pavel Sinitcyn, Georg H. H. Bornerthen washed three times in PBS. Nuclei were stained with DAPI (300 nM in PBS; Thermo Scientific Cat# 62248) for 5 min. Where indicated, coverslips were co-stained with DAPI and Alexa Fluor 488-labelled Phalloidin

(11) macroH2A2 antagonizes epigenetic programs of stemness in glioblastomaNature communicationsMay 27, 2023Nikolic A, Maule F, Bobyn A, Ellestad K, Paik S, Marhon SA, Mehdipour P, Lun X, Chen HM, Mallard C, et al.antibody (Invitrogen Goat anti-mouse IgG A568, A11011), and a DAPI counterstain (1:1000; Thermo Fisher #62248). Washes were performed using PBS with 0.1% Tween. Staining for FLAG was performed using an anti-FLAG

(12) Balancing WNT signalling in early forebrain development: The role of LRP4 as a modulator of LRP6 functionFrontiers in Cell and Developmental BiologyApril 7, 2023Shuang Geng, Fabian Paul, Izabela Kowalczyk, Sandra Raimundo, Anje Sporbert, Tamrat Meshka Mamo, Annette Hammes1:1,500; SOX2, rabbit, Abcam, ab97959, 1:100; GFP, chicken, Abcam, ab13970, IHC—1:200; DAPI, Invitrogen, 62248, 1:1,000; cleaved-Caspase-3, rabbit Cell Signaling CST #9661, 1:1,000. Secondary antibodies and final

(13) Proximity biotinylation to define the local environment of the protein kinase A catalytic subunit in adrenal cellsSTAR protocolsMarch 17, 2023Omar MH, Lauer SM, Lau HT, Golkowski M, Ong SE, Scott JD.Biomedicals,195368 Β-Glycerophosphate,Sigma,G6251 Cellstar Easystrainers 40 μm,VWR,89508-342 DAPI (1 mg/mL); 1:5000 dilution,Life Technologies,62248 TBST (10×),Cell Signaling Technologies,9997S Sodium hydroxide,Fisher,S318-500 Hydrochloric acid,Fisher,A144S-500 0.2

(14) Yiyi Fuzi Baijiang Powder Alleviates Dextran Sulfate Sodium-Induced Ulcerative Colitis in Rats via Inhibiting the TLR4/NF-κB/NLRP3 Inflammasome Signaling Pathway to Repair the Intestinal Epithelial Barrier, and Modulating Intestinal MicrobiotaOxidative medicine and cellular longevityJanuary 1, 2023Yang J, Miao L, Xue Y, Wang X.antibody (83383) were purchased from Cell Signaling Technology, Inc. (Boston, USA). ECL (A38555), DAPI (62248), and anti-NLRP3 antibody (PA5-79740) were purchased from Thermo Fisher Technology Co., Ltd. (Pittsburgh,

(15) Deletion of Tgfβ signal in activated microglia prolongs hypoxia-induced retinal neovascularization enhancing Igf1 expression and retinal leukostasisGliaSeptember 1, 2022Usui-Ouchi A, Eade K, Giles S, Ideguchi Y, Ouchi Y, Aguilar E, Wei G, Marra KV, Berlow RB, Friedlander M.were washed and suspended with 1 ml of 4% FBS/PBS containing DAPI (1:2000; Thermo Fisher Scientific, 62248) and DRAQ5 (1:5000; Cell signaling, 40845) for exclusion of dead cells and debris. We used clone RB6‐8C5

(16) Receptor activity-modifying protein 1 regulates mouse skin fibroblast proliferation via the Gαi3-PKA-CREB-YAP axisCell communication and signaling : CCSApril 12, 2022Yin S, Song R, Ma J, Liu C, Wu Z, Cao G, Liu J, Zhang G, Zhang H, Sun R, et al.Fluor-555 (#4413; diluted 1:200; CST) at room temperature for 1 h. Nuclei were stained with DAPI (#62248; Thermo Fisher Scientific) at room temperature for 30 min. Finally, the slides were covered with ProLong Gold antifade reagent

(17) Induction of SREBP1 degradation coupled with suppression of SREBP1-mediated lipogenesis impacts the response of EGFR mutant NSCLC cells to osimertinibOncogeneDecember 1, 2021Chen Z, Yu D, Owonikoko TK, Ramalingam SS, Sun SY.antibody was purchased from Cell Signaling Technology, Inc (#3662; Beverly, MA). Nile Red (N1142), DAPI (62248) and the secondary antibodies, Alexa Fluor 488-Donkey anti-mouse (A32766) and Alexa Fluor 568-Donkey

(18) A chemical genetic screen identifies Aurora kinases as a therapeutic target in EGFR T790M negative, gefitinib-resistant head and neck squamous cell carcinoma (HNSCC)EBioMedicineFebruary 1, 2021Low JL, Lau DP, Zhang X, Kwang XL, Rohatgi N, Chan JV, Chong FT, Wong SQR, Leong HS, Thangavelu MT, et al.blocking buffer followed by incubation with secondary antibodies and DAPI (Thermo Fisher Scientific, cat# 62248, 1:10,000) in PBS for 1 h at room temperature. Primary antibody used was anti-N-Cadherin (Cell Signalling

(19) Dissecting the heterogeneity and tumorigenesis of BRCA1 deficient mammary tumors via single cell RNA sequencingTheranosticsJanuary 1, 2021Sun H, Zeng J, Miao Z, Lei KC, Huang C, Hu L, Su SM, Chan UI, Miao K, Zhang X, et al.Cell Signaling Technology) were used for immunoflouresencent staining, and nuclei were stained with DAPI (62248; ThermoFisher Scientific). Analysis of public human breast cancer data We reanalyzed molecular data

(20) Tracking cells in epithelial acini by light sheet microscopy reveals proximity effects in breast cancer initiationeLifeJuly 21, 2020Alladin A, Chaible L, Garcia Del Valle L, Sabine R, Loeschinger M, Wachsmuth M, Hériché JK, Tischer C, Jechlinger M.Cat. # 61–7300, dilution 1:500). The nuclei were counter stained with 1:1000 DAPI (ThermoFisher Cat. # 62248, 1 mg/ml, dilution 1:1000) and mounted in anti-fading mounting medium (VECTASHIELD Mounting Medium with

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PTMScan ® Control Peptides Phospho-Akt (RXXS/T) from Cell Signaling Technology

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PTMScan ® Control Peptides Phospho-Akt (RXXS/T) from Cell Signaling Technology

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PTMScan ® Control Peptides Phospho-Akt (RXXS*/T*) from Cell Signaling Technology

Cell Signaling Technology

Citations (21)

Reviews

PTMScan ® Control Peptides Phospho-Akt (RXXS*/T*) from Cell Signaling Technology

PTMScan ® Control Peptides Phospho-Akt (RXXS/T) from Cell Signaling Technology

PTMScan ® Control Peptides Phospho-Akt (RXXS/T) from Cell Signaling Technology